Sentences with phrase «sebia electrophoresis»

Using the kits, you can quickly and easily label proteins in lysates or purified proteins with near - infrared CF ® dyes before electrophoresis and blotting.

Common tests are performed on large, highly sophisticated automated analysers, while more specialised tests are performed using technology such as absorption spectroscopy, electrophoresis, HPLC, GC - MS, and tandem MS, immunoassay, and DNA analysis.

Patent protection can also be sought for a new way to culture cells (a «method») or for a novel electrophoresis apparatus (a «machine»).

«Flow cytometry identified a large population of apoptotic melanoma cells, and single - cell electrophoresis indicated that mensacarcin causes genetic instability, a hallmark of early apoptosis,» Loesgen said.

The appearance of the variant after starch - gel electrophoresis of the hemolysates supports the concept that the lactic dehydrogenase isozymes are determined by the interaction of two subunits which are under separate genetic control.

This allows for the implementation of standard molecular biology tools such as genetic analysis (electrophoresis), amplification (PCR), sample preparation, and purification.

They come to the teaching lab at FHCRC and spend a day extracting DNA, micropipetting, and learning about gel electrophoresis.

The FEMTO Pulse, an automated pulsed - field capillary electrophoresis system, accurately sizes fragments as large as 165,000 bp and measures samples at concentrations as low as 5 fg / µL.

Runtimes are approximately one hour versus up to 16 hours needed for methods such as agarose - based, pulsed - field gel electrophoresis.

The NLISA platform uses a process call gel electrophoresis to screen synthesized, single strands of DNA «reagents» that change their shape in the presence of a specific biomarker.

In gel electrophoresis, an electric field pulls molecules through a porous gel.

In his spare time, he invented gel electrophoresis, one of the workhorse analytical tools of molecular biology.

In combination with gel electrophoresis, that shape change makes it extremely easy to detect whether or not the biomarker of interest is present.

Then, combining the nanoswitch reagents for PSA with the serum samples, they performed gel electrophoresis on the mixture.

By combining two chemical techniques — narrow capillary electrophoresis and mass spectrometry — Moini figured out how to measure that ratio in just 20 minutes using microscopic samples of silk.

Unusually short proteins can be distinguished from full - sized ones using gel electrophoresis, which separates molecules according to their size.

The method uses a well - established technique called immunoaffinity capillary electrophoresis (IACE).

Vijayvargia's team of master's degree students recently designed and built a DNA gel electrophoresis unit with power pack and ultraviolet illuminator that would cost as much as $ 2000 commercially; their version cost about $ 35.

The next step, microfluidic electrophoresis, uses an electrical current to move and further separate the P1 molecules from any other remaining components.

Conventional electrophoresis is performed in slabs of gel.

The ultimate goal is to put not just electrophoresis, but the preparation of DNA and the analysis of the DNA patterns all onto a single device controlled by a built - in computer.

Our new electrophoresis method allows us to increase the number of test samples on a given run by up to ten-fold and to complete the assay in less than half the time of the traditional comet process.»

The researchers created methods based on capillary electrophoresis to process soil or ice samples and detect 17 different amino acids simultaneously.

It miniaturises a technique called DNA electrophoresis.

But what about a band on an electrophoresis gel?

This advance allows the stationary metal electrodes fixed in electrophoresis equipment to be replaced.

Gel electrophoresis is a process through which different proteins or DNA fragments are separated so that they can be identified and studied.

We hope that this result will accelerate the automation and miniaturization of gel electrophoresis, which in turn can make medical diagnoses and DNA sequencing cheaper, faster, and more reliable,» says Assoc. Prof. Nathaniel Robinson, leader of the research group, on their «work as a logical extension of previous studies on conducting polymer electrodes in electrokinetic systems.»

After waiting for the chemical reactions to take place, we take my CRISPR plasmid to an electrophoresis machine, a tray that has wires hooked to it.

Although the primary effect of the electric field is cracking cell structures for better availability of nutrition, other effects like shockwave, electrophoresis or influence on the metabolic condition of cells can play a role.

After a few days, we isolate the DNA from the cells, amplify it with the polymerase chain reaction, and use electrophoresis to show that the CD32 gene has been cut into pieces.

We have compared the transcriptome in blood leukocytes, liver, and brain of humans, chimpanzees, orangutans, and macaques using microarrays, as well as protein expression patterns of humans and chimpanzees using two - dimensional gel electrophoresis.

The polypeptides are then subjected to two rounds of a routine separation technique called gel electrophoresis.

Typhoon supports phosphor imaging, 2D difference gel electrophoresis imaging, and RGB and NIR fluorescence, as well as sensitive and accurate quantitation of proteins, nucleic acids, and other biomolecules.

Researchers from the University of Granada (UGR) have used their own method of «microextraction and capillary electrophoresis» to analyse concentrations of a kind of mycotoxins, patulin, in 19 batches of eight brands of commercial apple juice.

Two - dimensional polyacrylamide gel electrophoresis combined with chromatographic phosphoprotein pre-enrichment and electrospray ionization mass spectrometry - based protein identification was used to reveal changes of phosphoproteins in cells exposed to cyclic stretching.

Amplified sequences were visualized by gel electrophoresis in 2 % agarose gels stained with GelRed (Biotium).

Electrophoresis was carried out for 16 h at 60 °C and 50 V in a linear 32 to 42 % denaturant (formamide) gradient.

The success of the PCR amplification was confirmed by electrophoresis and 5 uL of post-PCR reaction product was treated to degrade unused primers and nucleotides using USB ® HT ExoSAP - IT ® High - Throughput PCR Product Cleanup (Affymetrix, http://www.affymetrix.com) according to the manufacturer's instructions.

Primers were chosen from exons separated by large introns, when possible, and PCR reaction quality and specificity were verified by gel electrophoresis and melting - curve dissociation analysis of the amplified product.

Any technology that displaces capillary electrophoresis of STRs absolutely must provide CODIS profiles.

Two - Dimensional Gel Electrophoresis - Based Proteomic Analysis Reveals N - terminal Truncation of the Hsc70 Protein in Cotton Fibers In Vivo.

The products obtained were run on a PAGE (polyacrylamide gel electrophoresis) gel at 400 V for approximately 1.5 h.

Intact Golgi membranes were purified from Arabidopsis using density centrifugation and free - flow electrophoresis (FFE).

The 2 alleles differ by a single nucleotide, so restriction digestion of the amplified product followed by gel electrophoresis effectively differentiates the 2 alleles.

Clark's new techniques using capillary electrophoresis and microchip technology may allow for real - time field screening of plutonium and other materials by nuclear proliferation investigators and forensic scientists at the Department of Homeland Security.

Oliver Smithies was a prolific inventor and devised the method of using potato starch as medium for electrophoresis gel.

Microfabricated Channel Array Electrophoresis for Characterization and Screening of Enzymes Using RGS - G Protein Interactions as a Model System.

Gel electrophoresis was performed at 300mA current for 30 minutes at room temperature in dark.

The resulting DNA fragments are analyzed by agarose gel electrophoresis.