Capabilities in the cryo - EM Centre eBIC span
single particle analysis and molecular and cellular tomography using FEI technology and direct electron detectors.
Work by A.Z. and the development of the advanced
single particle analysis methods (A.Z.) were supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences, Division of Chemical Sciences, Geosciences, and Biosciences.
- EMAN2.2 for
Single Particle Analysis and In - situ Structural...
We will continue to study the relationship between the 3D structure and the function of macromolecular complexes using a combination of 3D Electron Microscopy, Cryo - Electron Tomography,
single particle analysis, correlative fluorescence microscopy, image analysis, and needed biochemical techniques.
Not exact matches
Analysis of seven
particles captured by aerogel and foil reveals diverse characteristics not conforming to a
single model.
«Most approaches to
single -
particle analysis have so far been based on trying to determine these
particle orientations from the images; however, the best resolution achievable from these
analyses is restricted by how precisely these orientations can be determined from noisy data.»
Single -
particle analysis also combines information from many
particles — whether proteins, complexes, or whole viruses — to generate a high - resolution, three - dimensional map.
But these models require computer «averaging» of data from
analysis of thousands, or even millions of like molecules, because it is so difficult to resolve the features of a
single particle.