«After our earlier publication on these samples, we switched from
amplicon to shotgun, with the intention
of simply getting larger sample
sizes,» said lead author Michael Tessler, a recent graduate
of the Museum's Richard Gilder Graduate School.
PCR
amplicons from COLQ exon 14 are 470 bp in
size and cleaved into 204 and 266 bp fragments in the presence
of c. 1010T > C. Three clinically normal littermates were identified as carriers, denoted by semi-filled icons.