Sentences with phrase «southern blot analysis»

We also recommend that recipients of ES cells re-verify the identity of the ES cell line by RT - PCR and / or Southern blot analysis (Genotyping BayGenomics Mice).

To further increase the sensitivity and specificity of the PCR assays, amplified gag and pol sequences were confirmed by Southern blot analysis using the biotinylated oligoprobes XGAGP2 (5» ACC TTG CAG CAC TGG GGA GAT GTC 3»), and XPOLP (5» TTG ATG AGG CAC TGC ACA GAG ACC 3») and chemiluminescence detection.

Southern blot analysis of the complemented clone, named ospC7 / ospC +4, demonstrated that it contained both WT and mutated copies of ospC on cp26 as well as the flgB p - aacC1 fusion downstream of the ospC gene (Fig. 2).

Relevant restriction sites used for cloning or for Southern blot analysis are indicated.

(B) Southern blot analysis of targeted cell lines.

The BIOMED - 2 multiplex PCR approach is a rapid and reliable procedure that is more sensitive than that of the Southern blot analysis in detecting clonality.

It seems that the Southern blot analysis can now be reliably replaced in a routine laboratory setting [6].

Although Southern blot analysis has proved to be a gold standard method in the detection of clonal rearrangement, it has several technical disadvantages, such as time necessary to obtain results, the use of radioactivity, and the susceptibility of the method to various artifacts [5].

BIOMED - 2 multiplex immunoglobulin / T - cell receptor polymerase chain reaction protocols can reliably replace Southern blot analysis in routine clonality diagnostics.

(B) Southern blot analysis of EcoRI - digested genomic DNA from wt, Xpd † XPCS / wt, and Xpd † XP / wt recombinant embryonic stem cell clones hybridised with the 3 ′ probe depicted in (A).

Additionally, southern blot analyses did not detect integration of plasmids in these clones (Fig. 3C).

Not exact matches

DNA methylation analyses using the bisulphite genomic sequencing method and Southern blotting indicated that the upstream boundary of the potential imprint may coincide with the 5» end of the upstream repeats.