For label -
free quantification analyzing unfractionated CSF samples (individual patient samples with insufficient volume (protein content) for immunoaffinity depletion and SCX fractionation), the LTQ - Orbitrap Velos mass spectrometer was operated in the
data - dependent mode with full scan MS
spectra (m / z 400 — 2000) acquired in the LTQ - Orbitrap Velos with resolution of 60,000 at m / z 400 (accumulation target: 1,000,000).