Sentences with phrase «spleen cell suspensions»

Consistent with this finding, enumeration of annexin V + DCs and active Caspase - 3 + DCs in spleen cell suspensions failed to show differences in their frequency in wild - type and EBI2 - deficient mice (not shown).

Single spleen cell suspensions from all three categories of mice were prepared in complete RPMI 1640 medium (CRPMI) containing 5 × 10 − 5 M 2mercaptoethanol (Sigma).

Spleen cell suspensions that were enriched or depleted of CD8 + cells were examined by flow cytometry for the relative percent of αβTCR +, γδTCR +, and Thy 1 + cells.

Spleen cell suspensions were collected 7 — 14 days after CD4 KO mice rejected BALB / c corneal allografts.

Not exact matches

Spleens were processed into cell suspensions through a 70 - μm strainer by a syringe plunger in RPMI 1640 with 10 % FBS and then treated with ACK lysis buffer to remove RBCs.

Spleens were excised and single cell suspensions were made by disruption of the spleen on wire mesh using the plunge of a 3 ml syringe.

Single - cell suspensions were obtained from spleen, mesenteric lymph node (MLN), and organs of mice and were stimulated in vitro with 50 ng / ml PMA and 500 ng / ml ionomycin (both from Sigma - Aldrich) for 5 — 6 h in the presence of GolgiStop or GolgiPlug (BD Biosciences).