Sentences with phrase «stem cell assays»

Stem Cell Assays is a collaborative project of stem cellresearchers and cell therapy experts aiming to promote the stem cell field.

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The findings from this one cell line are also inconsistent with clinical data from preimplantation genetic diagnosis, further suggesting that the stem cell assay may not wholly reflect what happens during human development.

Again, it is unclear how faithfully this stem cell assay recapitulates what would happen in the developing embryo.»

Not exact matches

The ability of SIF - seq to use reporter assays in mouse embryonic stem cells to identify human embryonic stem cell enhancers that are not present in the mouse genome opens the door to intriguing research possibilities as Dickel explains.

Along with intestinal stem cells, the team identified differentiated nutrient - digesting and absorbing enterocytes, mucus - producing Goblet cells, hormone - secreting enteroendocrine cells, and microbiome - regulating and sensing Paneth cells, and they performed a series of assays that confirmed their functions.

Next, because KLF4 is involved in self - renewal of stem cell, we also examined the effect of both miR - 7 and KLF4 on proliferation of CSCs by MTS assay and found that miR - 7 was capable of blocking the growth of CSCs, whereas ectopic expression of KLF4 significantly mitigated this effect of miR - 7 (Fig. 4C).

Phenotypic stability and unlimited self - renewal capacity of cell derived from pluripotent stem cells are essential properties for cell assay development for High Throughput Screening (HTS) strategies.

Methods: Human corneas with attached scleral rims were obtained from eye banks and were assayed for telomerase activity and BrdU (bromodeoxyridine) incorporation to determine, respectively, the presence of a stem - like cell marker and replicative activity.

All the hES and human induced pluripotent stem cell lines were confirmed to be genetically stable and pluripotent in in vivo and in vitro assays after extensive culturing on the new substrata.

Genetic approaches in mouse model systems combined with cell biological assays have allowed Professor Lukas Sommer to identify mechanisms regulating stem cell fates in the developing CNS and in neural crest - derived tissues.

This assay detects cells that are able to give rise to new colonies containing a majority of cells that express stem cell markers.

The majority of these assays were developed at the Columbia University Motor Neuron Center (MNC) and the Harvard Stem Cell Institute (HSCI).

These serial tumor transplantation experiments and replating assays provide concrete experimental evidence that PC3 holoclones contain stem - like tumor cells that could initiate serially transplantable tumors.

Overall, these results indicate that changes in ATP levels are a reliable indicator of cell death in stem cell populations upon drug insults and may have utility for hESC - based automatic screening assays.

It is tantalizing to speculate that amiodarone HCl might also be toxic to other stem cell populations that demonstrate increased ion channel expression relative to their differentiated derivatives, including mesenchymal stem cells (MSCs) and endothelial precursor cells [36], thus expanding the utility of the automated screening assay described here.

Remarkably, a single expanded potential stem cell can contribute both to the embryo proper and to the trophectoderm lineages in a chimaera assay.