Sentences with phrase «strand synthesis system»

For detection of XMRV gag sequences by nested RT - PCR, ∼ 200 ng of extracted RNA were first subjected to reverse transcription using the Superscript III First - Strand Synthesis System for RT - PCR (Invitrogen) according to the manufacturer's instructions.

cDNA was synthesized using the SuperScript III First - Strand Synthesis System for RT - PCR (Invitrogen).

cDNA was prepared from total RNA by reverse transcription using standard protocols for the SuperScript ® III First - Strand Synthesis System (Invitrogen) and oligo (dT) priming.