Sentences with phrase «synthetic signal transduction»

We describe assembly and function of a complete synthetic signal transduction pathway in plants that links input from computationally re-designed PBPs to a visual response.

We describe the production of transgenic plants using input from computationally re-designed receptors that signals through a synthetic signal transduction system.

Our synthetic signal transduction pathway is able to functionally link input from the computationally re-designed receptors to a plant response system, producing a prototype detector plant.

To determine if input from the PBP receptor could transcriptionally activate our synthetic signal transduction system, we produced plants containing: ssTNT.R3 → Fls - Trg - PhoR → PhoB - VP64 → PlantPho promoter:: GUS (Figure 1), hereafter called the complete signal transduction system.

Our synthetic signal transduction system was built using a modular assembly of bacterial and plant protein domains.

We show that evolutionarily conserved histidine kinase signal transduction components [9], [10], [11] can be used to build a synthetic signal transduction system, and we then use this system to link input from computationally re-designed receptors to a plant response.

In addition, because we developed PhoB - VP64 for plants by activating endogenous HKs with exogenous cytokinins [12], it is likely that our synthetic signal transduction system (receptor → HK → PhoB: VP64 → signal receptive promoter) has false input or cross-talk from plant HK components.

We then show that an adapted HK functions in plants by linking it to a transcriptional response through signal - dependent nuclear translocation of a bacterial response regulator [12], to produce a synthetic signal transduction system.

Citation: Antunes MS, Morey KJ, Smith JJ, Albrecht KD, Bowen TA, Zdunek JK, et al. (2011) Programmable Ligand Detection System in Plants through a Synthetic Signal Transduction Pathway.

Diagram of the complete synthetic signal transduction system in transgenic plants, using TNT as the ligand, signaling through the bacterial chimeric histidine kinase, and adapted response regulator, then activating a transcriptional response.

Ligand - dependent induction of our synthetic signal transduction system has stochastic fluctuations or noise.

Not exact matches

By functionally linking the signal transduction of melanopsin to the control circuit of the nuclear factor of activated T cells, we have designed a synthetic signaling cascade enabling light - inducible transgene expression in different cell lines grown in culture or bioreactors or implanted into mice.

Synthetic multivalent ligands as probes of signal transduction.

While the threshold - like behavior of the de-greening circuit likely contributes to these differences, we can not rule out the need to balance our synthetic HK signal transduction components for optimal transmission, in a manner similar to that in bacteria [31].

Initial screens showed that detached leaves of plants containing the synthetic sensing and signal transduction system linked to the de-greening circuit respond to the ligand (as low as 10 pM TNT or 2.3 ppt, parts per trillion).

Approaches successful in other systems, such as modeling signal transduction and synthetic biology methodologies [32] could improve future generations of detector plants by enhancing signal transmission and providing means to deal with biological «noise».

We previously showed that the bacterial response regulator PhoB [14], [15] can be adapted to function in a partial synthetic eukaryotic signal transduction system [12].