Sentences with phrase «t cell analysis»

Through patients» T cell analysis, Dr. Matsuzaki cloned and analyzed function of NY - ESO -1-specific CD8 + and CD4 + T cells.

After the 12 - week program, the researchers drew a second blood sample from each volunteer and ran the same T cell analysis.

Not exact matches

And a new analysis of the STEP trial, published last November in Proceedings of the National Academy of Sciences USA, provides a warning that the very vectors (adenoviruses, which are also employed in other vaccine development work) used to distribute the inactive HIV strains can actually make the immune system more vulnerable to infection by recruiting susceptible T cells to mucous membranes, where they are more likely to be infected during sexual activity.

Chemical analysis showed that its structure resembled that of a protein found on T cells.

And a new analysis of the stopped STEP trial, published online Monday in Proceedings in the National Academy of Sciences, provides a warning that the very vectors (adenoviruses, which are also employed in other vaccine development) used to distribute the inactive HIV strains can actually prime the immune system to be infected by recruiting susceptible T cells to mucous membranes, where they are more likely to be infected during sexual activity.

The analysis suggests that the degree of T - cell clone sharing between twins is greater than can be explained simply by shared genomes.

Single - cell differential gene expression analysis revealed a spectrum of known transcripts, including several linked to cytotoxic and costimulatory function that are expressed at higher levels in the TEMRA (effector memory T cells expressing CD45RA) subset, which is highly enriched for CD4 - CTLs, compared with CD4 + T cells in the central memory (TCM) and effector memory (TEM) subsets.

Simultaneous T cell antigen receptor (TCR) analysis in single cells and bulk subsets revealed that CD4 - TEMRA cells show marked clonal expansion compared with TCM and TEM cells and that most of CD4 - TEMRA were dengue virus (DENV)-- specific in donors with previous DENV infection.

Analyses of RV144 volunteers revealed that particular vaccine - induced immune responses, including production of certain antiviral antibodies and CD4 + T cell responses to HIV's outer shell, or envelope, correlate with reduced HIV infection.

Further analysis showed that wild type HIV - 1 tends to integrate itself into or near genes affecting CD4 + T cell activation and metabolism more frequently than does the mutant strain.

A series of biological analyses of the mice with Tim - 1 and immune cells isolated from human donors showed that Ebola virus directly binds to T - cells through Tim - 1 protein binding and causes massive inflammation that thwarts the immune system.

Their analysis showed that the diversity of T - cell receptors decreased among samples of T - cells taken closer to the tumor, compared with farther away.

(B, C) Flow cytometric analysis of HIV - 1 gene expression in (B) mock infected or (C) latently infected CD4 + T cells under non-polarizing conditions, either at the basal state or after reactivation with antibodies against CD3 and CD28.

X-ray studies at the Department of Energy's SLAC National Accelerator Laboratory, combined with Stanford biological studies and computational analysis, revealed remarkable similarities in the structure of binding sites, which allow a given T cell to recognize many different invaders that provoke an immune response.

At day 34 post infection, human CD4 + T cells were purified by positive selection prior to analysis to reduce any bias from low frequency contaminating human cells.

Analysis of antigens recognized on human melanoma cells by A2 - restricted cytolytic T lymphocytes (CTL).

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Molecular analyses revealed that some of the former regulatory T cells had become follicular helper T cells (Tfh).

This analysis showed differential expression associated with the haplotypes at both loci, suggesting the risk haplotypes are associated with an effect on T - cell response.

A collaborative group including the CRI Cancer Immunotherapy Consortium (CIC), the Association for Cancer Immunotherapy (CIMT) Immunoguiding Program (CIP), and scientists at Stanford University launch the Minimal Information About T cell Assays (MIATA) Project, the first systematic effort to establish a framework for reporting immune monitoring data from T cell assays used in clinical trials, with the goal of fostering reliability of data to facilitate meaningful interpretation, comparison, and meta - analysis across clinical trials.

Analysis of variant - specific antigens by selection of antigen - loss variants with cytolytic T cell clones.

Of note today: non-exclusive breastfeeding increases the risk of HIV transmission via the alteration of gut microbiome / T - cell activation; Fasting altered the gut microbiome in beneficial ways but only in mice previously fed a high fat diet; An investigation into new species of the honey and bumblebee gut commensal genus Gilliamella; Catfish development shapes gut microbial community structure independent of diet; A metagenomic analysis of the skin microbiome of the frog, Craugastor fitzingeri; The microbiome is altered during the bioremediation of herbicide contaminated soil; The impact of urban density on the soil microbiome; A randomized placebo controlled clinical trial of a microbiota based drug for the prevention of Clostridium difficile Infection; and the virome of the Cuatro Ciénegas Basin of Mexico

He then laid out his strategy for figuring out how to take advantage of the tumor - targeting T cells that patients already possess, and stressed the need for improved systems biology tools, including the ability to perform automated, high throughput analyses to perform deep interrogations into patients» T cell repertoires.

Consistent with previously reported analyses, we also observed enhanced IFN - γ and granzyme B production in DGKζ and Cbl - b — deficient T cells.

Infant T cell repertoire analysis combines quantitative PCR to determine relative frequency of T cell receptor chain families with deep sequencing (LINK TO URI GSC) of the CDR3 region to evaluate clonal diversity within each family.

An analysis of HIV vaccine trials (mostly ones that use DNA vaccines) showed that women showed better T - cell responses to such vaccines than men, and people with a lower body mass index showed better T - cell responses than people with a higher body mass index.

Cancer exome analysis reveals a T - cell - dependent mechanism of cancer immunoediting Nature DOI: 10.1038 / nature10755

Studies in mouse models will permit extension of the human study to parameters that can not be assessed with human samples, and enable selective analyses of antigen - specific T and B cells.

a Cellular composition analysis for 18 cell types shows an increase in NK - cells - resting and T - cells - CD8 and decrease in Neutrophils composition from pre - to post-mortem blood samples.

The program provides accurate, rapid genotyping and chimerism analysis; automatically identifies donor and recipient peaks in post-BMT samples, calculates percent chimerism and quality metrics for single donor or double donor cases, easily appends for longitudinal monitoring post-BMT, and has multi-lineage capabilities for chimerism analysis of T - cells, B - cells, and other cell type populations.

BIOMED - 2 multiplex immunoglobulin / T - cell receptor polymerase chain reaction protocols can reliably replace Southern blot analysis in routine clonality diagnostics.

Cell lysates were harvested and subjected to immunoblot analysis for MEK1, MEK2, MKK3, MKK6, phosphorylated ERK1 / 2 (p - ERK1 / 2), total ERK1 / 2 (t - ERK1 / 2), phosphorylated p38 (p - p38), total p38 (t - p38) and actin.

Dr. Sharpe's functional analysis of costimulatory pathways regulating T cell activation has led to understanding of (1) the roles of B7 - 1 and B7 - 2 as positive regulators through CD28 and (2) negative regulators through CTLA - 4, and (3) the role of PD - L1 and PD - L2 as negative regulators through PD - 1.

EBV - Associated T / NK — Cell Lymphoproliferative Diseases in Nonimmunocompromised Hosts: Prospective Analysis of 108 Cases.

In their latest study, researchers at La Jolla Institute for Allergy and Immunology (LJI) used single - cell transcriptome analysis to identify a hitherto unknown precursor for a poorly understood subgroup of killer T cells that is primarily found in humans with chronic viral infections.

Single cell analysis identifies new immune cell precursor; reveals surprising heterogeneity in a poorly understood subgroup of antiviral T lymphocytes

For Western blot assay and immunoprecipitation analysis, CD4 + T cells were isolated from mice spleen on day 21 after transplantation using a Mouse CD4 + T Cell Isolation Kit (Miltenyi Biotec), and the procedures were performed as described previously (35).

Precursors of human CD4 + cytotoxic T lymphocytes identified by single - cell transcriptome analysis.

(C and D) Flow cytometry analysis of intracellular IFN - γ and IL - 17A on donor - derived CD4 + T cells from spleen of control mice and VPA recipients on the indicated days.

(A and B) Flow cytometry analysis of intracellular IFN - γ and IL - 17A on donor - derived CD4 + T cells from spleen on indicated days for syngeneic and allogeneic mice.

Epigenomic analysis of primary human T cells reveals novel enhancers associated with Th2 memory differentiation and asthma susceptibility.

There will be a $ 10.00 extra charge for analysis of each additional cell by SKY, karyotyping by G banding, Sister Chromatid Exchange assay, scoring of Telomere - signal free ends and T - SCE.

Quantitative PCR analysis was performed to compare transcript levels of Venus with PrEn (Hex, Gata4, Gata6, Dab2, Sox7, Hnf4α, and Pdgfrα), pluripotency (Nanog, Klf4, Rex1, Stella, and Pou5f1) and other lineage (T, Fgf5, Eomes, Flk1, Mixl1, Cdx2, Sox1, Pax6, and Six3) markers in purified cell fractions.

Genetic Analysis of HIV Genomic DNA Sequences from Phenotypic and Antigen - specific CD4 + T Cell Subsets

A thorough longitudinal analysis of the CMV - specific T cells in nonagenarians showed a stable pattern with respect to frequency, phenotype, and clonal composition.

Comprehensive analysis of West Nile virus T cell responses in human infection.

Clonal analysis of the total CD8 T cell repertoire was compared between CMV - seropositive and CMV - seronegative donors.

We used direct sequence analysis to determine if the JAK2V617F mutation was present in acute myeloid leukemia (AML), chronic myelomonocytic leukemia (CMML) / atypical chronic myelogenous leukemia (aCML), myelodysplastic syndrome (MDS), B - lineage acute lymphoblastic leukemia (ALL), T - cell ALL, and chronic lymphocytic leukemia (CLL).

His research includes, uncovering the complex transcriptional network that exists in the tumor microenvironment roles of cancer initiating cells in immune suppresion, understanding the mechanism of T cell anergy and molecular analysis of T cell repertoire of patients with breast cancer.

Conclusions: In an analysis of peripheral blood mononuclear cells and intestinal tissues from patients with inflammatory bowel diseases vs controls, we found that reactivity to intestinal bacteria is a normal property of the human CD4 + T - cell repertoire, and does not necessarily indicate disrupted interactions between immune cells and the commensal microbiota.