Sentences with phrase «t cell donors»

Yu et al. (46) used mice deficient for both T - bet and RORγt as T cell donors and showed that blockade of both Th1 and Th17 differentiation is required to prevent acute GVHD.

Not exact matches

Potential donors must also have blood drawn to test for HIV, HTLV (human T - cell lymphotropic virus), Syphilis, Hepatitis B, and Hepatitis C.

To estimate the prevalence of positive serology among potential donors to a human milk bank.Retrospective review of our experience with donor serological testing at our milk bank over a 6 - year interval.Not - for - profit, regional human milk bank.Volunteer, unpaid potential donors of human milk.Serological testing for syphilis, HIV, hepatitis B, hepatitis C, human T cell lymphotropic virus type 1 (HTLV - 1) and human T cell lymphotropic virus type 2 (HTLV - 2).

Donors should be tested for HIV 1 and 2, Human T - cell lymphotropic virus or HTLV 1 and 2, syphilis, and hepatitis B and C.

Nearly half of 13 blood donors also had T cells that seek and destroy cells that make S. aureus Cas9 protein.

Now, by harnessing advances in genome editing to slice and dice genes in donor T cells, researchers have created a new type of cancer immunotherapy.

Through adoptive transfer, these mice then received donor T cells that had not been exposed to ovalbumin and therefore did not recognize it as a tolerable antigen.

When spleen CD4 + T - cells were transferred from heart - attack donor mice to naïve recipient mice, they induced long - term left ventricle dysfunction, fibrosis and enlargement, hallmarks of heart failure.

One was a bone marrow transplant, in which imported donor cells could manufacture healthy T cells to fight invading germs.

The idea was to see if those naive donor T cells would receive antigen - specific training from their pAPCs and then become tolerant to ovalbumin.

«We studied human T cells, isolated from blood donors of all ages, to compare mature cytotoxic T cells with naive ones,» said Philip Ansumana Hull, graduate student in Ott's lab and one of the first authors of the study.

Memory T cells against NS1 or E proteins were poorly cross-reactive, even in donors preexposed to DENV.

Simultaneous T cell antigen receptor (TCR) analysis in single cells and bulk subsets revealed that CD4 - TEMRA cells show marked clonal expansion compared with TCM and TEM cells and that most of CD4 - TEMRA were dengue virus (DENV)-- specific in donors with previous DENV infection.

Effector T cells incite GvHD when they become overactive as the patient's immune system starts to rebuild itself from the donor stem cells.

A series of biological analyses of the mice with Tim - 1 and immune cells isolated from human donors showed that Ebola virus directly binds to T - cells through Tim - 1 protein binding and causes massive inflammation that thwarts the immune system.

While the T cells of the liver transplant recipients reacted to the donor organ cells weakly, their reaction to other antigens was preserved.

Another approach to taming the donor graft relies on a mixture of anti — T cell anti-bodies called antithymocyte globulin, which is produced in horses or rabbits.

Given to the patient shortly before the transplant, the infusion of antibodies theoretically reduces the host's residual T cells, minimizing the risk of graft rejection while eliminating T cells from the donor to thwart GVHD.

In the current study Davis's group used this approach to determine the frequency of H - Y — specific T cells in a group of blood donors.

The researchers inject cells from the donor, so that T cells in the thymus are exposed to donor cells as well as «self» cells.

T cells normally attack tissue they do not recognise as «self,» causing organs which have been transplanted from other donors to be rejected.

That approach, which shuts down the hyperactive donor T cells when they first arrive, has not only allowed patients to tolerate grafts from increasingly mismatched hosts but, in several recent studies, has also cut rates of severe acute and chronic GVHD to less than 15 %.

Using a new method, the scientists therefore isolated T cells specifically programmed to target the CMV virus from the blood of the donor and transferred small numbers of these cells to the patients.

In further investigations of human liver cells from nearly 50 donor tissues of humans with varying degrees of body mass index (BMI) and liver fat, higher levels of CD8 + T cells were linked with higher levels of blood sugar or more advanced fatty liver disease.

To determine whether ZFN - mediated disruption of cxcr4 indeed protects CD4 + T cells from an in vitro HIV challenge, human CD4 + T cells from three different ccr5 wild type donors were stimulated and transduced with AdX4 - ZFNs or an AdR5 - ZFNs control.

If T - cells from a donor are used, the donor cells will view all of the patients» cells as foreign and attack them.

The Great Ormond Street team was able to use donor T - cells to treat Layla because in addition to the CAR gene, they used gene editing to disable the gene for the protein that recognises other cells as foreign, thereby preventing the donor cells from attacking Layla's healthy cells.

His team has used CRISPR gene editing to also disable the gene for the HLA proteins that mark a donor T - cell as foreign, reducing the chances of the recipient's immune system attacking the donor cells.

Even so, in many cases there is yet another hurdle to overcome, because the patient's own immune system will see the donor T - cells as foreign and kill them.

By contrast, DN T cells are able to mediate DTH to donor alloantigens and induce apoptosis of donor - specific corneal endothelial cells.

The capacity of CD8 − T cells from CD4 KO donors to mediate corneal allograft rejection is puzzling and on the surface, counterintuitive, since these cells are presumably double negative (DN) T cells.

In our study, CD8 − T cells from CD4 KO rejector mice failed to display CTL or DTH activity, yet they were capable of inducing donor - specific apoptosis of corneal endothelial cells.

The role of DN T cells in corneal allograft rejection was confirmed in two separate in vitro assays in which CD8 − cells were isolated from CD4 KO donors that had rejected corneal allografts and were found to induce apoptosis of donor - specific corneal cells.

However, lymphocytes isolated from recipients of either CD8 + T cells or CD8 − T cells produced significant in vitro apoptosis of donor - specific corneal endothelial cells.

It occurs when the donor T cells (the graft) don't recognize other cells in the recipient's body (the host) and attacks them as if they are foreign bodies.

We have also generated T - regs (CD4 + / 25high / 127low / --RRB- in vitro from donor AD - MSC and recipient peripheral blood mononuclear cells and these T - regs are infused in thymus of renal allograft recipients after kidney transplantation.

Use of a lower dose of rabbit anti — T - lymphocyte globulin (ATLG) was superior to a higher dose in children with hematologic malignancies undergoing allogeneic hematopoietic stem cell transplantation (HSCT) from an unrelated donor, according to the results of a study published in Lancet Oncology.

The program provides accurate, rapid genotyping and chimerism analysis; automatically identifies donor and recipient peaks in post-BMT samples, calculates percent chimerism and quality metrics for single donor or double donor cases, easily appends for longitudinal monitoring post-BMT, and has multi-lineage capabilities for chimerism analysis of T - cells, B - cells, and other cell type populations.

GVHD is an immune - mediated disease in which donor T cells recognize and attack the histocompatibility - disparate recipient; it involves multiple organs, such as the lung, liver, intestinal tract, and skin (2 — 4).

On day 0, recipient mice were transplanted with 5 × 106 T cell — depleted bone marrow (TCD - BM) cells and 1 × 106 spleen T cells from B6 donors.

In addition, we observed that donor T cells recovered from VPA - treated BMT recipients and controls on day 14 showed comparable cytolytic activity against host - type leukemia cells (Fig. 5F).

In addition, studies showed that the elimination of residual leukemia was primarily mediated by donor CD8 + cytotoxic T lymphocytes and NK cells, whereas the inflammatory cytokines that are secreted mainly by CD4 + Th cells have a very limited role in leukemia eradication but contribute significantly to the toxicity of GVHD (55).

University of Texas M.D. Anderson Cancer Center Tumor - specific alloantigen - anergic donor - derived T - cell therapy after hematopoietic stem - cell transplantation

Donor - type CD4 (+) CD25 (+) regulatory T cells suppress lethal acute graft - versus - host disease after allogeneic bone marrow transplantation.

(A) Donor - derived spleen T cells from VPA recipients and control mice on days 7, 14, and 28 were stained for Foxp3 expression.

We then treated BMT recipients with VPA or vehicle and recovered donor T cells from spleen and MLN of recipients on the indicated days after transplantation.

(F) Donor T cells were isolated from recipient spleen on day 14 for CTL assay against leukemia cells.

(B) Representative percentage of donor - derived spleen T cells expressing Foxp3 on days 7, 14, and 28.

(C and D) Flow cytometry analysis of intracellular IFN - γ and IL - 17A on donor - derived CD4 + T cells from spleen of control mice and VPA recipients on the indicated days.

These findings reveal a unique role for VPA as a histone deacetylase inhibitor in reducing the donor CD4 + T cells that contribute to GVHD, which may provide a strategy to reduce GVHD while preserving the GVL effect.