Sentences with phrase «t cells from»

Moreover, using a Bayesian deconvolution method to estimate methylation levels from the data [53], we found promoter methylation levels to be significantly inversely correlated (p value = 2.3e - 25) with previously published gene expression levels of T cells from human samples (Figure S1).

For comparison, researchers also checked B and T cells from 27 healthy children.

Although, remarkable progress has been made in the in vitro development of T cells from ES cells [6], [7], this has not been the case for other hematopoietic cell lineages.

Results: We apply a novel analytical approach to measure and compare transcriptional and epigenetic variability genome - wide across CD14 ⁺ CD16 ^- monocytes, CD66b ⁺ CD16 ⁺ neutrophils, and CD4 ⁺ CD45RA ⁺ naïve T cells from the same 125 healthy individuals.

We explored how aging affects transcriptional dynamics using single - cell RNA sequencing of unstimulated and stimulated naïve and effector memory CD4 (+) T cells from young and old mice from two divergent species.

Established a Jurkat cell model of HIV latency (5A8 cells) that is inducible by T - cell receptor cross-linking and displays drug reactivation profiles resembling that from resting CD4 T cells from aviremic patients.

H) Lastly, splenic CD4 + T cells from these tolerant animals were used as responder cells in an ELISPOT assay that measured IL - 2 production.

In patients with inflammatory bowel diseases, microbiota - reactive CD4 + T cells were reduced in the blood compared with intestine; T - cell responses that we detected had an increased frequency of interleukin 17A production compared with responses of T cells from blood or intestinal tissues of controls.

Results: Circulating and gut - resident CD4 + T cells from controls responded to bacteria at frequencies of 40 - 4000 per million for each bacterial species tested.

However, when the mice were reconstituted with T cells from aged donors, the expression of the V186.2 gene by young B cells was diminished and the response was dominated by the C1H4 gene, another member of the V186.2 / V3 family.

To examine the effect of age on the cognate function of CD4 T cells, we have used a novel adoptive transfer model that allows us to compare identical numbers of antigen - specific naive T cells from young and aged TCR transgenic (Tg) donors.

Here we show that the frequency of CD8 + T cells expressing the inhibitory killer cell lectin - like receptor G1 (KLRG1), a marker of cells unable to undergo further clonal expansion, was markedly elevated in CD8 + T cells from old donors.

A cell - intrinsic inhibitor of HIV - 1 reverse transcription in CD4 T cells from elite controllers.

Thus, mice reconstituted with T cells from the aged and B cells from the young had severely compromised mutational mechanism.

(A and B) Flow cytometry analysis of intracellular IFN - γ and IL - 17A on donor - derived CD4 + T cells from spleen on indicated days for syngeneic and allogeneic mice.

(E and F) Lethally irradiated BALB / c mice were transplanted with 5 × 106 TCD - BM plus 1 × 106 total spleen T cells or CD25 - depleted T cells from B6 mice.

To identify unique or redundant roles for gamma (c) cytokines in naive CD4 (+) T cells, we compared monoclonal populations of CD4 (+) T cells from TCR - Tg mice that were gamma (c)(+), gamma (c)-LRB--), CD127 -LRB-- / --RRB- or CD122 -LRB-- / --RRB-.

(C and D) Flow cytometry analysis of intracellular IFN - γ and IL - 17A on donor - derived CD4 + T cells from spleen of control mice and VPA recipients on the indicated days.

We then treated BMT recipients with VPA or vehicle and recovered donor T cells from spleen and MLN of recipients on the indicated days after transplantation.

Researchers at Oxford have demonstrated that autophagy is diminished in T cells from aged mice, and T cell responses could be boosted in older mice using the autophagy - inducing compound spermidine.

(A) Donor - derived spleen T cells from VPA recipients and control mice on days 7, 14, and 28 were stained for Foxp3 expression.

Adoptive T cell transfer is removing T cells from the patient, genetically modifying or treating them with chemicals to enhance their activity, and then re-introducing them into the patient.

We isolated spleen CD4 + T cells from VPA recipients and vehicle recipients and assessed the phosphorylation level of Akt.

Although mice deficient in BTLA do not show lymphocyte developmental defects, T cells from these animals are hyperresponsive to anti-CD3 Ab stimulation.

On day 0, recipient mice were transplanted with 5 × 106 T cell — depleted bone marrow (TCD - BM) cells and 1 × 106 spleen T cells from B6 donors.

This suggests that treatment with IL - 15 can successfully relieve tumor - resident killer T cells from functional suppression and reactivate them to kill pancreatic cancer cells.

Using this model, they confirmed that beta - catenin - expressing cancers did prevent T cells from entering the tumor microenvironment.

Educating T cells from the cradle through adolescence

In late 2015, the company also launched its MagCloudz streptavidin cell - separation kit and partnered with the University of Massachusetts Medical School in research toward the enrichment and purification of CD3 + T cells from human umbilical cord blood.

Drug delivery guru Daniel Anderson of Massachusetts Institute of Technology points out that one of the most advanced programs is Sangamo Biosciences» ongoing clinical trial to remove T cells from patients, edit their DNA to make them resistant to HIV, and reinject the modified cells.

Peptide stimulation of T cells from Cbl - b − / − infected mice were similar in their ability to produce IFN - γ relative to WT controls (Fig. 5D), as previously seen in mice injected with a low dose of LCMV Docile (37).

To define the signaling changes resulting from each genotype, we isolated naive CD8 + T cells from mice deficient in DGKζ, Cbl - b, or both in DKO; stimulated the cells through their TCRs; and evaluated phosphorylation of Erk and phosphorylation and degradation of IκBα.

T cells from mouse splenocytes were purified with magnetic naive CD8 + T cell isolation kits using instructions provided by the manufacturer (Stemcell Technologies, Vancouver, Canada).

Purified T cells from the spleen were labeled with CellTrace CFSE (Invitrogen, Carlsbad, CA) using protocols and reagents supplied by the manufacturer.

Nippostrongylus brasiliensis can induce B7 - independent antigen - specific development of IL -4-producing T cells from naive CD4 T cells in vivo.

Moreover, we found that T cells from double - knockout (DKO) mice did not demonstrate enhanced tumor activity above that observed in DGKζ single - knockout (KO) mice.

As has been noted by others (35), T cells from infected DGKζ − / − or DKO mice demonstrated enhanced IFN - γ production after stimulation with LCMV - specific peptides gp33 and np396 (Fig. 5D), and T cells from infected DGKζ − / − mice demonstrated enhanced IFN - γ production after stimulation with LCMV - specific peptide gp276 relative to T cells from WT mice (Fig. 5D).

Additional studies are attempting to determine if the transgene expression of various anti-apoptotic genes can protect T cells from tumor - induced apoptosis.

BALB / c corneal allografts were transplanted to C57BL / 6 beige nude mice that received either CD8 − or CD8 + T cells from C57BL / 6 CD4 knockout (KO) mice that had rejected BALB / c corneal allografts.

More specifically, we found that T cells from DGKζ − / − and Cbl - b − / − mice exhibit similar levels of enhanced proliferation at low levels of TCR stimulation.

To determine if this was also true for Cbl - b − / − and DKO T cells, we isolated naive CD8 + T cells from mice deficient in DGKζ, Cbl - b, or both, prior to stimulation with anti-CD3 (Fig. 3D — F).

Both CD8 − and CD8 + T cells from CD4 KO corneal allograft rejector mice mediated corneal allograft rejection following adoptive transfer to nude mice.

The extensive apoptosis of allogeneic corneal endothelial cells by CD8 − T cells from CD4 KO mice was perplexing, as this cell population should not contain CD4 + cells.

Exhausted CD39 + CD8 + T cells from mice hydrolyzed extracellular ATP, confirming that CD39 is enzymatically active.

Indeed, our results indicate that adoptive transfer of CD8 + T cells from CD4 KO mice that had rejected corneal allografts resulted in the rejection of 95 % of the corneal allografts transplanted to athymic recipients.

The CD8 + T cells were already primed, as they were collected from CD4 KO mice that had rejected corneal allografts and thus, may have functioned differently from CD8 + T cells from naïve CD4 KO mice.

Although T cell receptor engagement was sufficient to induce CD39 on human CD8 + T cells, exposure to IL - 6 and IL - 27 promoted CD39 expression on stimulated CD8 + T cells from human or murine sources.

In our study, CD8 − T cells from CD4 KO rejector mice failed to display CTL or DTH activity, yet they were capable of inducing donor - specific apoptosis of corneal endothelial cells.

Steven A. Rosenberg and colleagues show that bulk T cells transduced with T cell receptor genes are used to treat patients with melanoma, bypassing the need to expand tumor - specific T cells from patients with cancer.

The capacity of CD8 − T cells from CD4 KO donors to mediate corneal allograft rejection is puzzling and on the surface, counterintuitive, since these cells are presumably double negative (DN) T cells.