In our study we invsetigated CD4 +
T cells sorted from spleens of infected mice.
CD4 +
T cells sorted by immunomagnetic beads from splenocytes of animals tolerant to donor - type cardiac allografts were plated at 106 cells / well.
Not exact matches
When dendritic
cells not only signal myelin as a «target substance,» but also secrete the messenger substance IL - 6, a
sort of molecular switch is flipped in the
T cells.
But before immune - system fighters called
T cells will attack foreign tissue, they must first get a confirmation order of
sorts: a costimulatory signal.
The research provides a
sort of rulebook that can be used with high success to track down antigens likely to activate a given
T cell, easing a bottleneck that has constrained such studies.
Naïve CD4 +
T cells were isolated by MACS microbead negative
sorting using the naïve
T cell isolation kit (Miltenyi Biotec).
Because the treatment uses the patient's immune
cells as a
sort of
T -
cell training force, it is an immunotherapy.
FITC + LC / DC
sorted from the LNs of lymphoid deficient or control mice stimulated naive
T cells with similar efficiency.
When absolute purity is not necessary, as is often the case with in vitro stimulation of
T cells, magnetic
cell separation can deliver highly enriched
cells faster, with significant cost - savings, and without exposure to harsh separation protocols like flow cytometric
sorting, or chemical gradients.