To assess low frequency disruption events, we conducted 454 deep sequencing on all 15 sites in both control CD4 + Tcells and those treated with X4 - ZFNs, yielding approximately 7,500 — 26,000 reads per site in the ZFN - treated samples (Table S2).
Vaccines that aim to expand tumor - specific CD8 (+) Tcells have yielded disappointing results in cancer patients although they showed efficacy in transplantable tumor mouse models.