Sentences with phrase «t7 endonuclease»
The resulting fragments were digested by T7 endonuclease I (NEB).
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Before in vitro transcription, we linearized the plasmids with appropriate restriction endonucleases and generated sense and antisense riboprobes using in vitro transcriptions (Roche Applied Science) with T7 polymerase and the DIG RNA labeling kit (Roche Diagnostics).