• ArcherDX, Inc., a Boulder, Colo. - based company focused on NGS - based
gene fusion detection assays, raised $ 35 million in Series A preferred funding.
Because of a chromosomal abnormality, this gene sometimes fuses with another gene and forms a hybrid, or
a gene fusion, resulting in a gene product with an entirely different function, causing cancers.
«We found targetable FGFR
gene fusions across a diverse array of cancer types.
Studies in prostate tissues show that
the gene fusion almost always indicates cancer.
This gene fusion, T2: ERG, is believed to cause prostate cancer.
After a median follow - up of 11 months, 11 of the 13 patients who responded remain on the study, including one patient who had non-small cell lung cancer (NSCLC) with a ROS1
gene fusion who has had a complete response that has been maintained for more than two years.
«We look forward to conducting further research through the STARTRK - 2 phase II trial and are hopeful that treatment with entrectinib in patients with a range of advanced or metastatic solid tumors harboring NTRK1 / 2/3, ROS1, or ALK
gene fusions will result in very meaningful benefit.»
The researchers found abnormal fusion - circular RNAs (f - circRNAs), corresponding to different exons associated with the PML - RARα
gene fusion as well as the MLL - AF9
gene fusion.
Although BRAF
gene fusions were well known to be involved in PLGGs, the study team found important differences in a related gene, CRAF, in which mutations can also lead to this type of glioma.
«Biology of childhood brain tumor subtypes offers clues to precision treatments: Researchers reveal differences among
gene fusions in low - grade pediatric brain tumors.»
Future researchers could apply this technique to efficiently deliver large repair templates for applications that require large
gene fusions.
The same effect was seen in a mouse model of human brain cancer containing
this gene fusion.
At the time, it was thought that
this gene fusion was limited to a fraction of brain tumors, affecting about 300 patients in the U.S. per year.
Sequencing accelerates the analysis of cancer associated alterations in genome sequence such as insertions and deletions, CNVs, inversions, duplications, translocations and
gene fusions, as well as single nucleotide variants.
«It's unlikely that we will find
a gene fusion responsible for most glioblastomas.
Drs. Iavarone, Lasorella, and Rabadan and CUMC have filed a patent application related to the diagnostic and therapeutic use of FGFR - TACC
gene fusions.
«It's probably the single most common
gene fusion in human cancer,» said study co-leader Antonio Iavarone, MD, professor of neurology and of pathology and cell biology (in the Institute for Cancer Genetics) at CUMC.
Using a variety of experimental techniques, the researchers determined that
the gene fusion initiates a cascade of events that increases mitochondrial activity.
Dr. Iavarone and his colleagues suspected that glioblastomas might be addicted to proteins produced by
gene fusions.
These drugs are now being tested in patients with recurrent glioblastoma that contains
the gene fusion by one of the paper's co-authors, Marc Sanson, MD, of Pitié Salpêtrière Hospital in Paris.
Since then, however, other researchers have observed the same
gene fusion in a percentage of lung, esophageal, breast, head and neck, cervical, and bladder cancers, affecting tens of thousands of cancer patients overall.
This testing showed the presence of PDGFRB
gene fusions within the B - other group, particularly EBF1 - PDGFRB in about one - third of B - other ALL cases.
Hur is now exploring ways to use genetic engineering to target
gene fusion events that underlie some cancers.
Synovial sarcoma is a rare soft tissue sarcoma characterized by a t (X; 18) translocation, which results in a SYT - SSX
gene fusion.
By engineering an artificial protein that targets the product of such potentially cancer - causing
gene fusions, Hur hopes to trigger immune defense mechanisms that kill rogue cells harboring the fusions.
Also, genes that code for receptor tyrosine kinases, a family of receptors on the surface of cells, may rearrange to form multiple distinct
gene fusion partners, as evidenced in an article by Kulkarni, et al, on a translational study involving a patient who developed a BRAF fusion following treatment with a BRAF inhibitor1.
Therefore, strategies for understanding mechanisms of resistance and determining how to therapeutically target specific
gene fusion partners are also being explored.
New coding genes can arise in genomes through several processes, including gene duplication,
gene fusion, de novo formation from non-coding DNA, or lateral gene transfer (LGT) from another species.
Pathogen, salicylic acid and developmental dependent expression of a beta -1,3-glucanase / GUS
gene fusion in transgenic tobacco plants
One intriguing bit of work that Dr. Ley described was on the «Mouse APL» project, a knock - in mouse with the PML - RARA
gene fusion backcrossed 10 + generations to CBL / BL6 mice.
The main databases devoted stricto sensu to cancer cytogenetics are the «Mitelman Database of Chromosome Aberrations and
Gene Fusions in Cancer» (http://cgap.nci.nih.gov/Chromosomes/Mitelman), the «Atlas of Genetics and Cytogenetics in Oncology and Haematology» (http://atlasgeneticsoncology.org), and COSMIC (http://cancer.sanger.ac.uk/cosmic).
Molecular profiling of large tumour numbers has also allowed the annotation of more than 13 million non-coding mutations, 18 029
gene fusions, 187 429 genome rearrangements, 1 271 436 abnormal copy number segments, 9 175 462 abnormal expression variants and 7 879 142 differentially methylated CpG dinucleotides.
MyAML is a targeted panel that analyze the coding and non-coding exons of nearly 200 genes along with breakpoint hotspots within 36 of these genes identified in somatic
gene fusions.
GRM1 is upregulated through
gene fusion and promoter swapping in chondromyxoid fibroma Nature Genetics, online 23 March 2014, doi: 10.1038 / ng.2927
Not exact matches
Our chromosome 2 is a
fusion of two independent chromosomes found in the extant apes (that is, they bear the exact same
genes in the exact same order, etc).
Such methods include cell
fusion, microencapsulation and macroencapsulation, and recombinant DNA technology (including
gene deletion,
gene doubling, introducing a foreign
gene, and changing the positions of
genes when achieved by recombinant DNA technology).»
This microscopic image of fibroblast cells shows the induction of cell
fusion by a newly described
gene and its protein, called myomerger.
The current study indicates that myomerger is the missing link for
fusion, and that both
genes are absolutely required for
fusion to occur, according to the researchers.
Researchers then tested cell cultures and mouse models by using a
gene editing process called CRISPR - Cas9 to demonstrate how the presence or absence of myomaker and myomerger — both individually and in unison — affect cell
fusion and muscle formation.
He and his colleagues identified novel
fusions of the
gene FGFR2 in the tumors of four patients recruited to MI - ONCOSEQ.
Novel abnormalities in the FGFR
gene, called FGFR
fusions, were identified in a spectrum of cancers, and preliminary results with cancer cells harboring FGFR
fusions suggested that some patients with these cancers may benefit from treatment with FGFR inhibitor drugs, according to data published in Cancer Discovery, a journal of the American Association for Cancer Research.
In the steps of sperm - egg
fusion, IZUMO1 on the spermatozoon and the IZUMO1 counter-receptor on the egg, JUNO, play crucial roles in the very first step, the formation of an intercellular bridge, and deletion of either
gene leads to the failure of gamete membrane
fusion.
In 2011, Science's editors will be watching a smaller detector at the Large Hadron Collider called LHCb, which will study B mesons in great detail; new techniques that should lead to the discovery of many more
genes contributing to adaptation; an ignited
fusion burn at the National Ignition Facility; broadly neutralizing antibodies, which are capable of disabling a wide range of viral variants; the first plug - in hybrid electric cars whose batteries are charged from a wall socket go on the market; and the results of the first phase III trial of a malaria vaccine.
In 2011, Science's editors will be watching the Large Hadron Collider, adaptation
genes, laser
fusion, broadly neutralizing antibodies, electric cars, and malaria shots.
Subsequent procedures included mammalian somatic cell nuclear transfer, cell
fusion, induction of pluripotency by ectopic
gene expression, and direct reprogramming.
«We were surprised to find that mutations in this
gene also affect
fusion of autophagic organelles,» said Upasana Gala, a graduate student in the Program in Developmental Biology at BCM in Bellen's laboratory.
My background has included: PCR,
gene synthesis, cloning, codon optimization, site - directed mutagenesis, chromosomal
gene integration / deletion, albumin
fusion, fermentation using NBS BioFlo3000, SDS - PAGE, Coomassie staining, and Western blotting.
Computational genomics includes: bio-sequence analysis,
gene expression data analysis, phylogenetic analysis, and more specifically pattern recognition and analysis problems such as
gene finding, motif finding,
gene function prediction,
fusion of sequence and expression information, and evolutionary models.
Curious about the possibility of circular RNAs contributing to cancer, Pandolfi and his colleagues set out to see if they could detect relevant changes in tumors known to harbor distinct
fusion proteins, which result when different chromosomes abnormally join together, melding two separate
genes into a new centaur - like
gene.
(Normally, multiple circular RNAs can be generated from a single
gene, so it is not entirely surprising to find different f - circRNAs emerging from the same
fusion gene.)