Sentences with phrase «tag codons»

Isaacs and his colleagues have so far winnowed the 32 strains down to four, in which nearly all TAG codons are replaced by TAA codons.

Imagine the E. coli genome as a pizza cut into 32 slices that together contain all 314 TAG codons, but not on one slice.

Not exact matches

They are starting with the rarest, the stop codon TAG, which appears 314 times.

Theoretically, once a particular codon — say, TAG — has been removed from a genome, the cell's protein - making machinery could be reprogrammed to assign TAG to an amino acid, instead of being a stop signal.

The researchers sifted through the E. coli genome and identified all 314 TAG stop codons.

This approach will allow you, for example, to insert gene tags, loxP sites for conditional models and stop codons in specific functional regions.

The GFP - tagged isoform a (plasmid pCK28 -LCB- PW01A8.1:: W01A8.1 (a) synth:: gfp:: unc - 54 3 ′ UTR -RCB--RRB- was constructed by synthesizing the W01A8.1 a sequence with modified codons to allow protection from CRISPR / Cas9 targeted sgRNA and prepared as a GeneArt ® Strings ™ DNA Fragment from Invitrogen (Invitrogen, Carlsbad, California, USA) and cloned using GeneArt ® Seamless Cloning System (Invitrogen) into pPD95.75 (NeoR).