The pathways that connect expression of stem cell surface glycoconjugates such as
the TRA -1-60 / GCTM - 2 antigen, receptors, and growth factors in human and even mouse ES cells with the transcriptional networks that regulate pluripotency remain unclear.
(C — D) Immunofluorescence staining for (C)
TRA -1-60 and (D) TRA -1-81 on HUF1 cells.
We also found expression of a wide panel of markers expressed in hESCs in our iPS cells, which include OCT3 / 4, SSEA - 3,
TRA -1-60, TRA -1-81, NANOG, KLF4, and LIN28 (Figure 2E — M).
In this case, the gold nanoparticles are conjugated with antibodies against SSEA - 5 or
TRA -1-60, proteins that are found on the surfaces of stem cells.