Sentences with phrase «tissue analysis by»

Tissue analysis by collaborators at Purdue University revealed that, after its midbrain expression, the protein was capable of reaching nerve endings in the gastric wall.

Not exact matches

Right now such cancers are usually confirmed by way of a biopsy — a doctor literally removes tissue and sends it to a lab for analysis, which can take days.

By examining tissue from different disease stages within valve samples of the same patient, the researchers were able to avoid donor variation in their analysis.

The genetic analysis was made possible by tissue samples available through the Total Cancer Care ® Protocol at Moffitt.

In an analysis of Medicare billing data submitted by more than 2,300 United States physicians, researchers have calculated the average number of surgical slices, or cuts, made during Mohs micrographic surgery (MMS), a procedure that progressively removes thin layers of cancerous skin tissue in a way that minimizes damage to healthy skin and the risks of leaving cancerous tissue behind.

A postmortem analysis of human brain tissue, for example, conducted by Witelson and her colleagues at the Michael G. DeGroote School of Medicine at McMaster, revealed that women's neurons were 11 percent denser than men's in the prefrontal cortex and in a region of the temporal cortex that is involved with language processing, comprehension, and memory.

The scientists hope that publication of the protocol will also lead to an exchange with and further developments by colleagues with a view to advancing metabolic analyses of archived tissues.

Analysis of images showed that the contrast used by the research team bound almost exclusively to the fibrin - fibronectin complexes, producing a strong and prolonged image enhancement of micrometastases and tumors compared with normal tissue.

To help identify genes and signaling pathways that may be influenced by zinc, Knoell and his team performed a genome - wide microarray analysis of lung tissue taken from zinc - deficient mice with sepsis.

The Inquiry into Human Tissue Analysis in U.K. Nuclear Facilities was launched in 2007 and led by lawyer Michael Redfern.

A new genetic analysis reveals that our brawny cousins had a number of distinct genes involved in the buildup of certain types of fat in their brains and other tissues — a trait shared by today's Europeans, but not Asians.

Patients with recurrent glioblastoma multiforme (GBM) treated with an experimental vaccine made from the patient's own resected tumor tissue showed an improved survival compared with historical patients who received the standard of care alone, according to an analysis of a phase 2 trial of this vaccine that was recently published in the journal Neuro - Oncology and accompanied by an editorial highlighting the importance of the trial.

Their analysis is designed to help surgeons minimize the tissue removed while operating on cancer patients by providing highly accurate, real - time information about the health of the excised tissue.

Gross and histopathological analyses of post-mortem tissues were performed by a board - certified veterinary pathologist specializing in nonhuman primate / laboratory animal medicine, a branch of Primate Services at the CNPRC.

Mice were sacrificed by CO2 asphyxiation, and SVCs from perigonadal adipose tissue were collected for FACS analysis.

They recently conducted a feasibility study with promising results that have been published in an article in Medical Image Analysis called: «Integrated local binary pattern texture features for classification of breast tissue imaged by optical coherence microscopy.»

Tissues to be analyzed by FACS were processed immediately; other samples were frozen in liquid nitrogen and stored at — 75 °C prior to RNA extraction and immunohistochemical analysis.

The expression of a few retinal mRNAs known to be induced by hypoxia, including HIF - 1α, was assessed by qRT — PCR analysis of total RNA isolated from retinal tissue following exposure to 3 h of 6 % — 7 % O2.

Proteomics (Fagerberg et al, «Analysis of the human tissue - specific expression by genome - wide integration of transcriptomics and antibody - based proteomics») with a focus in the article on the tissue - specific pattern in the human body.

The exploration of the therapeutic potential of stem cells requires the characterization of their biological properties, the deciphering of the mechanisms that underlie their pluripotency and their capacity at differentiation, by the understanding of the signals that direct their fate towards discrete cell phenotypes... Their therapeutic use requests further, in particular in the case of substitutive therapies, the analysis of their capacities of integration in injured adult tissues and of their potential tumorigenicity, as well as the development of original ways of delivery.

Adding this extra information, as with utilising phase information or tissue - specific transcripts, increases the challenges for data processing and interpretation by adding complexity to the treatment of variant annotation, but with good reason: this added complexity reflects the underlying biology, so taking this information into account potentially adds significant value to analyses of DNA variants.

Computerized image analysis of cell - cell interactions in human renal tissue by using multi-channel immunoflourescent confocal microscopy Proc.

«We mapped the metabolic changes caused by accumulated fat in liver cells, and combined this data with an analysis of biological networks of liver and other human tissues.

Visualization and analysis of gene expression in tissue sections by spatial transcriptomics Patrik L. Ståhl, Fredrik Salmén, Sanja Vickovic, Anna Lundmark, José Fernández Navarro, Jens Magnusson, Stefania Giacomello, Michaela Asp, Jakub O. Westholm, Mikael Huss, Annelie Mollbrink, Sten Linnarsson, Simone Codeluppi, Åke Borg, Fredrik Pontén, Paul Igor Costea, Pelin Sahlén, Jan Mulder, Olaf Bergmann, Joakim Lundeberg, Jonas Frisén.

Analysis of the human tissue - specific expression by genome - wide integration of transcriptomics and antibody - based proteomics.

In their analyses, higher expression was more common in the distant sites, and this was confirmed by our studies, but unlike our series, that series included only one patient with both tissue types available.

Similarly, analysis of splicing changes with PMI did not show conclusive evidence of systematic splicing deregulation (as measured by the splicing entropy) across tissues.

We find that many genes change expression over relatively short PMIs in a tissue - specific manner, but this potentially confounding effect in a biological analysis can be minimized by taking into account appropriate covariates.

Research Focus: I am highly trained in producing and purifying monoclonal antibodies from Hybridoma cells, performing ELISA assays, preparing primary cells from organs and analyzing cell populations using FlowJo Cytometry data analysis software, isolating genomic DNA from animal tissues, maintaining and screening numerous transgenic and knock out mice lines by PCR and FACS.

The study by Louie Yang (Dept. Entomology at UC - Davis) used stable hydrogen isotope analysis to test tissue samples from 114 butterflies at 4 overwintering locations.

All samples under analysis in this study were collected and preserved with the PAXgene Tissue preservation system developed by Qiagen21.

Organization and evolution of brain lipidome revealed by large - scale analysis of human, chimpanzee, macaque, and mouse tissues.

In summary, no significant differences were identified between treated and untreated animals when the following were tested (by Chi squared analysis and Fisher's exact test): (1) Number of animals positive for B. burgdorferi by PCR; (2) number of animals positive for B. burgdorferi by xenodiagnosis; and (3) number of animals positive for B. burgdorferi by RT - PCR, either directly or by analysis of in vivo culture tissue.

The metabolic analysis of the mice could be combined with clinical assessments, by performing biochemical analysis in blood, plasma, urine and tissues (i.e, lipid and cholesterol content, glycogen content...) and by performing autopsy of the animal at the end of phenotyping study with several tissue collections (i.e, histology, mRNA anlaysis, protein analysis...).

Although applications involving use of deidentified human tissue were considered nonclinical applications in this analysis, it is possible that the definition of clinical research used by Williams identified a different subset of applications than were analyzed, even in the specific categories segregated in the current study.

Susan Amara, USA - «Regulation of transporter function and trafficking by amphetamines, Structure - function relationships in excitatory amino acid transporters (EAATs), Modulation of dopamine transporters (DAT) by GPCRs, Genetics and functional analyses of human trace amine receptors» Tom I. Bonner, USA (Past Core Member)- Genomics, G protein coupled receptors Michel Bouvier, Canada - Molecular Pharmacology of G protein - Coupled Receptors; Molecular mechanisms controlling the selectivity and efficacy of GPCR signalling Thomas Burris, USA - Nuclear Receptor Pharmacology and Drug Discovery William A. Catterall, USA (Past Core Member)- The Molecular Basis of Electrical Excitability Steven Charlton, UK - Molecular Pharmacology and Drug Discovery Moses Chao, USA - Mechanisms of Neurotophin Receptor Signaling Mark Coles, UK - Cellular differentiation, human embryonic stem cells, stromal cells, haematopoietic stem cells, organogenesis, lymphoid microenvironments, develomental immunology Steven L. Colletti, USA Graham L Collingridge, UK Philippe Delerive, France - Metabolic Research (diabetes, obesity, non-alcoholic fatty liver, cardio - vascular diseases, nuclear hormone receptor, GPCRs, kinases) Sir Colin T. Dollery, UK (Founder and Past Core Member) Richard M. Eglen, UK Stephen M. Foord, UK David Gloriam, Denmark - GPCRs, databases, computational drug design, orphan recetpors Gillian Gray, UK Debbie Hay, New Zealand - G protein - coupled receptors, peptide receptors, CGRP, Amylin, Adrenomedullin, Migraine, Diabetes / obesity Allyn C. Howlett, USA Franz Hofmann, Germany - Voltage dependent calcium channels and the positive inotropic effect of beta adrenergic stimulation; cardiovascular function of cGMP protein kinase Yu Huang, Hong Kong - Endothelial and Metabolic Dysfunction, and Novel Biomarkers in Diabetes, Hypertension, Dyslipidemia and Estrogen Deficiency, Endothelium - derived Contracting Factors in the Regulation of Vascular Tone, Adipose Tissue Regulation of Vascular Function in Obesity, Diabetes and Hypertension, Pharmacological Characterization of New Anti-diabetic and Anti-hypertensive Drugs, Hypotensive and antioxidant Actions of Biologically Active Components of Traditional Chinese Herbs and Natural Plants including Polypehnols and Ginsenosides Adriaan P. IJzerman, The Netherlands - G protein - coupled receptors; allosteric modulation; binding kinetics Michael F Jarvis, USA - Purines and Purinergic Receptors and Voltage-gated ion channel (sodium and calcium) pharmacology Pain mechanisms Research Reproducibility Bong - Kiun Kaang, Korea - G protein - coupled receptors; Glutamate receptors; Neuropsychiatric disorders Eamonn Kelly, Prof, UK - Molecular Pharmacology of G protein - coupled receptors, in particular opioid receptors, regulation of GPCRs by kinasis and arrestins Terry Kenakin, USA - Drug receptor pharmacodynamics, receptor theory Janos Kiss, Hungary - Neurodegenerative disorders, Alzheimer's disease Stefan Knapp, Germany - Rational design of highly selective inhibitors (so call chemical probes) targeting protein kinases as well as protein interaction inhibitors of the bromodomain family Andrew Knight, UK Chris Langmead, Australia - Drug discovery, GPCRs, neuroscience and analytical pharmacology Vincent Laudet, France (Past Core Member)- Evolution of the Nuclear Receptor / Ligand couple Margaret R. MacLean, UK - Serotonin, endothelin, estrogen, microRNAs and pulmonary hyperten Neil Marrion, UK - Calcium - activated potassium channels, neuronal excitability Fiona Marshall, UK - GPCR molecular pharmacology, structure and drug discovery Alistair Mathie, UK - Ion channel structure, function and regulation, pain and the nervous system Ian McGrath, UK - Adrenoceptors; autonomic transmission; vascular pharmacology Graeme Milligan, UK - Structure, function and regulation of G protein - coupled receptors Richard Neubig, USA (Past Core Member)- G protein signaling; academic drug discovery Stefan Offermanns, Germany - G protein - coupled receptors, vascular / metabolic signaling Richard Olsen, USA - Structure and function of GABA - A receptors; mode of action of GABAergic drugs including general anesthetics and ethanol Jean - Philippe Pin, France (Past Core Member)- GPCR - mGLuR - GABAB - structure function relationship - pharmacology - biophysics Helgi Schiöth, Sweden David Searls, USA - Bioinformatics Graeme Semple, USA - GPCR Medicinal Chemistry Patrick M. Sexton, Australia - G protein - coupled receptors Roland Staal, USA - Microglia and neuroinflammation in neuropathic pain and neurological disorders Bart Staels, France - Nuclear receptor signaling in metabolic and cardiovascular diseases Katerina Tiligada, Greece - Immunopharmacology, histamine, histamine receptors, hypersensitivity, drug allergy, inflammation Georg Terstappen, Germany - Drug discovery for neurodegenerative diseases with a focus on AD Mary Vore, USA - Activity and regulation of expression and function of the ATP - binding cassette (ABC) transporters

By SNP analysis, single nucleotide differences between the sequences of 22Rv1 - associated XMRV and XMRV genomes detected in prostate cancer tissues [VP35, VP42, and VP62 (2006)-RSB-(red lollipops) are corrected by the deep sequencing coverage data (black lollipopsBy SNP analysis, single nucleotide differences between the sequences of 22Rv1 - associated XMRV and XMRV genomes detected in prostate cancer tissues [VP35, VP42, and VP62 (2006)-RSB-(red lollipops) are corrected by the deep sequencing coverage data (black lollipopsby the deep sequencing coverage data (black lollipops).

New computational strategies are supported by our single cell data analysis experts with computational methods deconvolute tissue composition, determine cell type heterogeneity, identify novel cell type defining markers or track transcriptional dynamics.

Indeed, 30 % of all transcripts identified by cap - analysis of gene expression (CAGE) tags derived from human embryonic tissues have been associated with repetitive elements, and 16 % are retrotransposons [14].

These findings underscore the difficulty of detecting non-cultivable B. burgdorferi following antibiotic treatment, as interpretation of xenodiagnosis depends upon timing and number of ticks tested, and the outcome of infection must be measured by analysis of multiple tissues at appropriate intervals.

For canonical tissues, Seurat may be unbounded in terms of scalability and, Regev said «Drop - Seq is its best friend, «as parts of the Seurat package and work by Rahul Satija were integral for the analysis of cells and classification of the retina data obtained using Drop - Seq.

The results obtained by quantitative PCR for the genes tested were consistent with the results using RNA - seq (cosine correlation = 0.89; P < 0.001), thus providing a validation of the analysis (SI Appendix, Table S5 lists full list of tissues and comparisons).

Tissues were analyzed... the endpoints include (g / 100g) moisture, fat, protein, ash, carbohydrates... Protocol was based on established methodology by the Association of Official Analytical Chemist's (AOAC) Official Methods of Analysis (AOAC, 2000) and is briefly described herein.

Diagnosis can be confirmed by biopsy for bacterial cultures and tissue analysis.

The diagnosis of the TVT was further supported by the identification and analysis of long interspersed nuclear elements (LINE) from paraffin - embedded tumor tissue.

Definitive diagnosis is based on laboratory analysis of affected tissues by fluorescent antibody techniques.

[28] At the risk of appearing to be overly semantic about this analysis, I take it that what counsel want Dr. Malcolm to be able to do is to testify by way of opinion about whether or not there has been some form of harm or damage to the tissues of the brain of Ms. Meghji as opposed to some form of harm or damage to the mind or emotions or personality of Ms. Meghji.

(ii) Except as permitted by paragraph (f)(2)(i) of this section, the covered entity may not disclose for the purposes of identification or location under paragraph (f)(2) of this section any protected health information related to the individual's DNA or DNA analysis, dental records, or typing, samples or analysis of body fluids or tissue.

A phlebotomist is a specialized medical assistant that collects blood and other tissue samples to be used for analysis by laboratory workers.