Sentences with phrase «treatment of these cell lines»

Not exact matches

Senator Bernie Sanders (I — VT) has said he believes stem - cell research «represents an exciting and promising line of research that could provide treatments and cures for many debilitating diseases.»

Figure on the left: Analyzing the relative sensitivity of hundreds of cancer cell lines to commonly used anti-cancer drugs (each dot) reveals that cells with suppressed expression of one proteasome cap subunit are specifically resistant to treatments with the proteasome inhibitors MG132 and bortezomib.

«Considering that PDPN is associated with poor prognosis in GBM, CAR T - cell therapy that targets this protein is promising for treatment of patients with relapsed or resistant tumors following first - line chemotherapy,» says Toshihiko Wakabayashi, a coauthor and the chair of Department of Neurosurgery Nagoya University School of Medicine.

Human placental cell line Jeg - 3 can be readily infected by a circulating strain of Zika virus (left panel) and completely protected by treatment with nanchangmycin (right panel).

During lab testing, the compound «markedly inhibited» the growth of three human pancreatic cancer cell lines five days after treatment and induced the death of pancreatic cancer cells.

Research based on such false cell lines would undermine the understanding of different cancers and possible treatments, and clutter the scientific literature with bogus conclusions.

«Most researchers have relied on cell lines to screen the effect of drugs and other treatments including viruses,» said Dr. Cripe, who also is a professor at The Ohio State University College of Medicine.

Seven years after Moore's initial therapy, Golde asked his patient to sign a contract relinquishing his rights and those of his heirs to any cell lines or products derived from his blood or tissues (Moore's spleen had been removed in the course of his treatment).

Proponents of ESC research counter that most of the new cell lines could be derived from donated, unused human embryos created by couples seeking fertility treatment, and that more than a third of zygotes fail to implant after conception, so those would be lost by chance anyway.

The researchers showed that cabazitaxel worked better than docetaxel in human prostate cancer cells lines that were resistant hormone treatment, both in terms of slowing cancer - cell growth and in its ability to kill cancer cells.

British newspapers reported this weekend that Ian Wilmut, the University of Edinburgh biologist who led the team that in 1997 cloned Dolly the sheep, is getting out of the cloning business in light of the new findings, which seem to offer researchers a likely new source of stem cell lines for basic research that could one day lead to new treatments and perhaps cures for spinal injuries, diabetes and debilitating disorders such as multiple sclerosis and Parkinson's disease.

The researchers then exposed cells from each of these lines to a panel of 31 different drug treatments — including 23 chemotherapy compounds approved by the FDA for breast and ovarian cancers, six targeted cancer drugs, and two common drug combinations.

The resulting «map» of gene - drug interactions allowed the researchers to accurately predict the responses of multiple human cancer cell lines to different chemotherapy agents based on the cell lines» genetic profiles and also revealed new genetic factors that appear to determine the response of breast and ovarian tumor cells to common classes of chemotherapy treatment.

The idea is to collect enough cell lines to customize treatments to the immune system of any possible patient, he says.

«Although it's still early days for this line of research, these findings provoke the hypothesis that MHCII + innate lymphoid cells may be an important pathway to therapeutically target in the treatment of some chronic inflammatory diseases,» suggests Sonnenberg.

The research, the team said, should not discourage the pursuit of experimental treatments but instead be heeded as a call to screen rigorously all cell lines for mutations at various stages of development as well as immediately before transplantation.

Some ethicists suggest that tissue donors deserve a share of the tremendous commercial potential of iPS cell lines as disease models, drug - testing platforms or treatments.

One is a relatively unknown company, CyThera, set up less than 2 years ago in San Diego, that claims to have nine embryonic stem cell lines and is currently trying to develop pancreatic islet cells for treatment of diabetes.

By conducting basic research in the same cell lines that will be used in patients, researchers should also be able to speed the translation of their discoveries to treatments, says Crook.

In addition to validating these cells, the group has determined the sensitivity of these cell lines to a variety of chemotherapeutic agents currently in use and proposed for use in the treatment of canine cancers.

To see if any of these lay in genetic sequences on which the p53 protein acts, the investigators used data from several lines of healthy and cancerous cells subjected to various p53 - activating treatments.

In the original agreement, WARF gave Geron exclusive rights to develop diagnostic and treatment products in six types of stem cell - derived cell lines: pancreatic, heart muscle, nerve, blood, bone, and liver.

«In the future we are hopeful that BH3 - mimetics may be a valuable new line of treatment for Legionella and other bacteria that similarly hide out within cells,» Dr Vince said.

Individual cultures of 3T3 (a murine fibroblast line) and endothelioma cells subjected to identical treatment were used for negative and positive controls, respectively.

To determine whether the reactivation of methylation - silenced genes by EGCG is a general phenomenon that also occurs in other cell lines, we studied the effects of EGCG treatment on the methylation status and mRNA levels of p16INK4a or RARβ in three other human cancer cell lines (Fig. 3C) ⇓.

A Phase 3 Open - Label Randomized Study of Quizartinib (AC220) Monotherapy Versus Salvage Chemotherapy in Subjects with FLT3 - ITD Positive Acute Myeloid Leukemia (AML) Refractory To or Relapsed After First - line Treatment With or Without Hematopoietic Stem Cell Transplantation (HSCT) Consolidation

The FDA granted accelerated approval to avelumab (Bavencio, EMD Serono, Inc.), a checkpoint immunotherapy that targets the PD - 1 pathway, as a first - line treatment for a rare form of skin cancer called Merkel cell carcinoma (MCC).

To create cell models for the discovery of disease etiology, progression, and treatment, isogenic cell lines can be created from healthy or sick individuals, then compared (Figure 1).

First - and second - line treatment of clear cell RCC has changed dramatically, Dr. Eric Jonasch said during the NCCN Annual Conference in Orlando.

We report the presence of cancer stem cell like Side Population and their survival and enrichment post treatment with bleomycin in HCT116 cell line.

Treatment of lung cancer cell lines with mebendazole caused mitotic arrest by depolymerization of tubulin, followed by apoptotic cell death.

Treatment of the Karpas 299 and SU - DHL1 cell lines with increasing concentrations of rapamycin, an inhibitor of the mTOR - raptor complex, resulted in a marked concentration - dependent decrease of the phosphorylation levels of mTOR, p70S6K, 4E - BP1, and total eIF4E (Fig. 4A).

Of note, treatment of ALCL cell lines with rapamycin also resulted in a slight decrease of Ser473 phosphorylation of AKOf note, treatment of ALCL cell lines with rapamycin also resulted in a slight decrease of Ser473 phosphorylation of AKof ALCL cell lines with rapamycin also resulted in a slight decrease of Ser473 phosphorylation of AKof Ser473 phosphorylation of AKof AKT.

Investigators from Cedars - Sinai and Emulate demonstrated how cells of a human intestinal lining created outside an individual's body mirror living tissue when placed inside Emulate's Intestine - Chips, opening the door to personalized testing of drug treatments.

Treatment of the Gb3 - expressing gastric carcinoma cell line St3051 with STxB coupled to SN38, the active metabolite of the topoisomerase type I inhibitor irinotecan, resulted in > 100-fold increased cytotoxicity, as compared with irinotecan alone.

This is in accordance with previous reports that decitabine and 5 - azacytidine produce a marked synergistic effect in combination with suberoylanilide hydroxamic acid and romidepsin in T - lymphoma cell lines by modulating cell cycle arrest and apoptosis.26, 27 As a mechanism of action, KMT2D mutations of B - lymphoma cells promote malignant outgrowth by perturbing methylation of H3K4 that affect the JAK - STAT, Toll - like receptor, or B - cell receptor pathway.28, 29 Here our study indicated that dual treatment with chidamide and decitabine enhanced the interaction of KMT2D with the transcription factor PU.1, thereby inactivating the H3K4me - associated signaling pathway MAPK, which is constitutively activated in T - cell lymphoma.13, 30,31 The transcription factor PU.1 is involved in the development of all hematopoietic lineages32 and regulates lymphoid cell growth and transformation.33 Aberrant PU.1 expression promotes acute myeloid leukemia and is related to the pathogenesis of multiple myeloma via the MAPK pathway.34, 35 On the other hand, PU.1 is also shown to interact with chromatin remodeler and DNA methyltransferease to control hematopoiesis and suppress leukemia.36 Our data thus suggested that the combined action of chidamide and decitabine may interfere with the differentiation and / or viability of PTCL - NOS through a PU.1 - dependent gene expression program.

The AUGMENT treatment uses energy - producing mitochondria from your EggPC cells, which are immature egg cells found in the protective lining of your ovaries.

Therefore, it is necessary to establish multiple independent iPS cell lines from individual donors to ensure efficient generation of various types of functional cells for future application in the treatment of diseases.

Furthermore, using a phospho - tyrosine [Y] 474 (in - house generated) and a phospho - serine [S] 176 (Cell Signaling, Inc., Danvers, MA) RIPK2 antibodies, we confirmed that active RIPK2 is recognized by these antibodies upon activation with MDP treatment in 293HEK fibroblast and HCT116 colon cancer epithelial cells (Fig. 3E, left panel) and detection of constitutively active RIPK2 in the colon cancer cell line SW480 (Fig. 3E, right panCell Signaling, Inc., Danvers, MA) RIPK2 antibodies, we confirmed that active RIPK2 is recognized by these antibodies upon activation with MDP treatment in 293HEK fibroblast and HCT116 colon cancer epithelial cells (Fig. 3E, left panel) and detection of constitutively active RIPK2 in the colon cancer cell line SW480 (Fig. 3E, right pancell line SW480 (Fig. 3E, right panel).

Protein and mRNA for GHRH and GHRH - R were expressed in both HCC1806 and MX - 1 human TNBC cell lines as determined after five weeks of treatment using qRT - PCR.

Beyond the expansion of the collection of barcoded cell lines for testing larger panels of compounds, next steps will include determining whether PRISM can be used to study the complex interactions between different cell types found within tumors, or to study tumor evolution during cancer development or after cancer treatment.

The response of LIG4 (LIGIV) and PRKDC (DNA - PKc)-LRB-- / --RRB- cell lines to treatment with Etoposide was studied.

This effect on cell survival was not endothelial cell - specific, since IC50 values for kahweol treatment of several human tumoral cell lines were similar to those obtained for HUVEC (results not shown).

With about 100 lines of code, a Morgridge Institute for Research team has unleashed a fast, simple and predictive text - mining tool that may turbo - charge big biomedical pursuits such as drug repurposing and stem cell treatments.

This line of investigation could eventually lead to artificial blood vessels, grown with patients» own cells or «off the shelf,» or biological / pharmaceutical treatments that promote the regeneration of damaged blood vessels.

«The TAILOR study draws out attention to the fact that outcomes are poor in second - line treatment of non — small - cell lung cancer, with our available treatments resulting in median progression - free survivals of two or three months,» said Benjamin Solomon, MBBS, PhD, of Peter MacCallum Cancer Centre in Melbourne.

Stem cell lines grown in the lab provide scientists with the opportunity to «engineer» them for use in transplantation or treatment of diseases.

This lining consists of a layer of epithelial cells that come together to form a barrier that is impenetrable to the nanoparticles, thereby necessitating the use of injections to be an effective form of treatment.

Finally, analysis of tumors from clinical trials and cell line models will allow discovery of mechanisms of sensitivity and resistance to AR and PI3K inhibitors, as well as biomarkers that can be used in the development of new treatment regimens and selection of patients for future trials.

Acute myeloid leukemia (AML) is the leading cause of leukemia mortality in the United States.1 Curative treatment involves intensive induction chemotherapy, before proceeding to either consolidation chemotherapy or allogeneic stem cell transplantation based on the patient's risk for relapse.2 This approach has been employed for > 4 decades and, although most individuals achieve complete remissions with front - line therapy, 3 the majority of patients ultimately relapse with drug - resistant disease, and overall survival rates remain disappointingly poor.4 The limited ability of many patients to tolerate the intense chemotherapy - based treatments, in particular hematological toxicity, further contributes to the poor outcomes noted in this disease.