Sentences with phrase «tubulin antibody»
Immunohistochemistry of paraffin - embedded human brain tissue slide using 66240 -1-Ig (beta Tubulin antibody at dilution of 1:400 (under 10x lens)
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Immunocytochemistry / Immunofluorescence - Anti-alpha Tubulin antibody [DM1A]- Loading Control (ab7291)
Immunohistochemistry of paraffin - embedded human breast cancer tissue slide using 66240 -1-Ig (beta Tubulin antibody at dilution of 1:400 (under 10x lens)
Immunohistochemistry (Formalin / PFA - fixed paraffin - embedded sections)- Anti-alpha Tubulin antibody [DM1A]- Loading Control (ab7291)
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Immunofluorescent analysis of MDCK cells using 66200 -1-Ig (acetylated Tubulin (Lys40) antibody) at a dilution of 1:50 and Alexa Fluor 488 - conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
The membrane was blocked with 3 % BSA / PBS for 1 h at RT followed by incubation with the corresponding primary antibody in 1 % BSA / PBS - T overnight at 4 °C: pTyr (Millipore, # 05 - 321, 1:500), Tie2 (R&D, #AF313, 1:1,000), Tie2 (R&D, #AF762), pAKT (Cell Signaling, # 4060S, 1:1,000), AKT (Cell Signaling, # 9272S, 1:1,000), FOXO1 (Cell Signaling, # 9454, 1:1,000), pFOXO3A (Cell Signaling, # 9466, 1:1,000), FOXO3A (Cell Signaling, # 12829, 1:1,000), VEGFR2 (Cell Signaling, # 2479, 1:1,000), tubulin (Sigma, #T8203, 1:5,000).
Alpha tubulin (red) was detected using the mouse monoclonal (ab7291) antibody.
The cells were then incubated with the antibody ab16056 at 1µg / ml and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control) at 1/1000 dilution overnight at +4 °C.
After blocking overnight at 4 ° in 5 % non-fat dry milk in PBS / 0.1 % Tween - 20, blots were probed with the appropriate primary antibody for Keap1 (Cell Signaling), Nrf2 (Santa Cruz Biotechnology), or β - Tubulin (Sigma Aldrich).
Antibodies used for Western blotting included cleaved caspase - 3, phosphorylated CrkL (Y207), phosphorylated Akt (Ser473), Akt, TAZ, YAP1, phosphorylated STAT5 (Tyr694), STAT5, and ERBB2 from Cell Signaling; β - tubulin and actin from Sigma - Aldrich; ABL2 (9H5) from Santa Cruz Biotechnology; ABL1 (8E9) from BD Biosciences; IL - 6, TNC, and phosphorylated YAP1 (Tyr357) from Abcam; and MMP1 from Calbiochem.
Keiran's image shows the morphology of the neuron as visualised by staining with an antibody against bIII - tubulin (blue).
After scanning, the membranes were stripped and reprobed for α - tubulin using a mouse monoclonal antibody (Sigma - Aldrich).
Acknowledgments: We thank A. Miyawaki for kikGR, R. Renkawitz - Pohl for the antibody against β - tubulin; P. McCrea for the antibody against β - catenin, A. Altenburger for brachiopod data; S. Kaul - Strehlow, G. Mayer, M. V. Sørensen, and K. Worsaae for insightful discussions; I. Haußer - Siller at the Electron Microscopy Core Facility (EMCF)(BioQuant, Heidelberg University); and the EMBL EMCF.
The following primary antibodies were used: Oct - 4 (19857 Abcam) 1 ∶ 1000; β - III tubulin clone SDL.3 D10 (T8660 Sigma) 1 ∶ 500; Nestin (611658 BD Transduction laboratories) 1 ∶ 500 and TH (P40101 Pel - Freez) 1 ∶ 500, and as secondary antibodies: Alexa Fluor 594 Goat Anti-Mouse, Alexa Fluor 488 Goat Anti-Rabbit, Alexa Fluor 594 Goat Anti-Rabbit.