Sentences with phrase «tubulin in»

The labelling is for acetylated tubulin in red (identifying all axons), and green for the cell permeable dye calcein, which is only applied on the axonal side of the chambers (top half) and allows the identification of those neuronal cell bodies (bottom half) that have extended axons to the other side of the microfluidic device.

Ab7291 staining alpha tubulin in human breast cancer cell line by ICC / IF (Immunocytochemistry / Immunofluorescence).

ab7291 staining alpha - Tubulin in Caco - 2 cells.

Alpha tubulin is not suitable as a loading control in adipose tissue as expression of tubulin in adipose tissue is very low (Spiegelman and Farmer, Cell, 1982, 29 (1): 53 - 60, «in cells undergoing adipose differentiation actin synthesis decreases by 90 %»).

It does not inhibit polymerisation or depolymerisation of platelet tubulin in vitro.

ab7291 staining alpha Tubulin in SV40LT - SMC cells.

IHC image of ab7291 staining alpha Tubulin in rat colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond.

Microtubule dynamics and distribution of γ - tubulin in male germ cells of Bombyx mori (Lepidoptera).

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon.

Not exact matches

This unique organization of tubulin is preserved among all living plant and animal cells, because it is essential for way in which microtubules assemble, Al - Bassam said.

Al - Bassam said the results open up new ways of thinking about tubulin, tubulin - related disorders and molecular biology in general.

For example, formation of the mitotic spindle significantly depletes the numbers of free tubulin molecules in the soluble phase of the cytoplasm.

Which of these becomes dominant depends on the relative concentrations of the tubulins and the motor proteins: In a certain concentration range the dynamic equilibrium between growth and shrinkage of the microtubules operates as it would if resources were not limiting.

In this situation, the microtubules continue to grow until the concentration of tubulins falls below a critical value.

Even small defects in the genes that assemble tubulin are associated with serious developmental disorders, such as Kenny - Caffey syndrome and Giant Axonal Neuropathy.

Microtubules, hollow fibers of tubulin protein only a few nanometers in diameter, form the cytoskeletons of living cells and play a crucial role in cell division (mitosis) through their ability to undergo rapid growth and shrinkage, a property called «dynamic instability.»

Tubulin and tubulin - like proteins are highly conserved evolutionarily, found in some bacteria, fungi, higher plants, and aTubulin and tubulin - like proteins are highly conserved evolutionarily, found in some bacteria, fungi, higher plants, and atubulin - like proteins are highly conserved evolutionarily, found in some bacteria, fungi, higher plants, and animals.

It's also the first structure determined of any ligase in the tubulin tyrosine ligase - like (TTLL) family.

AHERF Neurosciences Grant (G9410 -020-Y01)- Co-PI; «Differential expression and sorting of the neuron - specific class III b - tubulin isotype in human neuronal and neuroendocrine tumor cell lines maintained in an organ culture system».

In the center, FGFR - TACC fusion protein (red) can be seen disrupting tubulin bundles (green), structures that support cell division, or mitosis, at the point connecting the two daughter cells (whose nuclei are colored blue).

We observed a modest decrease in cell cycling and some induction of the neuronal marker beta - III tubulin, as quantified here.

In vivo analysis of the functions of γ - tubulin - complex proteins.

This finding is consistent with the recent discovery with several «eukaryotic» signature genes in these archaeal lineages, including genes encoding proteins such as actin, tubulin, and several proteins involved in transcription and membrane deformation.

Here, FGFR - TACC (shown in red) can be seen interacting with tubulin bundles (green), structures that support mitosis, at the point connecting the two daughter cells (whose nuclei are colored blue).

One reaction serves as a positive control to amplify a portion of the tubulin gene found in all plants, and the other assays for genetic modification.

Chromosome segregation in fission yeast with mutations in the tubulin folding cofactor D. Current genetics, 50 (5), 281 — 294.

Vimentin filaments support extension of tubulin - based microtentacles in detached breast tumor cells.

ARHGAP21 Protein, a New Partner of α - Tubulin Involved in Cell - Cell Adhesion Formation and Essential for Epithelial - Mesenchymal Transition.

The membrane was blocked with 3 % BSA / PBS for 1 h at RT followed by incubation with the corresponding primary antibody in 1 % BSA / PBS - T overnight at 4 °C: pTyr (Millipore, # 05 - 321, 1:500), Tie2 (R&D, #AF313, 1:1,000), Tie2 (R&D, #AF762), pAKT (Cell Signaling, # 4060S, 1:1,000), AKT (Cell Signaling, # 9272S, 1:1,000), FOXO1 (Cell Signaling, # 9454, 1:1,000), pFOXO3A (Cell Signaling, # 9466, 1:1,000), FOXO3A (Cell Signaling, # 12829, 1:1,000), VEGFR2 (Cell Signaling, # 2479, 1:1,000), tubulin (Sigma, #T8203, 1:5,000).

«Though it has long been suspected in the field and there was indirect evidence to support the theory, this is the first time it has been shown directly, through live imaging, that IFT does function as a tubulin pump.

Specific interaction of benzimidazole anthelmintics with tubulin: high - affinity binding and benzimidazole resistance in Haemonchus contortus.

ICC / IF image of beta Tubulin staining in HeLa cells.

Immunofluorescent imaging of human cells (U2OS) with ab7291 reveals a delicate network of alpha - tubulin (green) located exclusively in the cytoplasm.

In cerebellar cultures from neonatal rats at postnatal day 1 (P1), approximately 90 % of the total cells were small neurons stained by anti-Tuj1 (neuron specific β3 - tubulin).

The cells were then incubated with ab7291 at a working concentration of 0.5 μg / ml and ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin (Alexa Fluor ® 647, shown in red) at 1/250 overnight at +4 °C, followed by a further incubation at room temperature for 1h with an anti-mouse AlexaFluor ® 488 (ab150117) at 2 μg / ml (shown in green).

Another post-translational modification of detyrosinated alpha tubulin is C - terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

After blocking overnight at 4 ° in 5 % non-fat dry milk in PBS / 0.1 % Tween - 20, blots were probed with the appropriate primary antibody for Keap1 (Cell Signaling), Nrf2 (Santa Cruz Biotechnology), or β - Tubulin (Sigma Aldrich).

We will use as an example the quantification of tubulin polyglycylation in wild - type testes and in Bug22 mutant testes, which present defects in the deposition of this posttranslational modification.

Inclusion Criteria: • Availability of tumor tissue for mesothelin expression testing • Histologically - confirmed, mesothelin - expressing metastatic or advanced non-metastatic disease (tumour type specific inclusion criteria) • At least one measurable lesion according to either Response Evaluation Criteria in Solid Tumors (RECIST) 1.1 or International Thymic Malignancy Interest Group (ITMIG) modified RECIST 1.1 as applicable • Adequate bone marrow, liver, renal and coagulation function • Left ventricular ejection fraction (LVEF) ≥ 50 % of the lower limit of normal (LLN) according to local institutional ranges • Eastern Cooperative Oncology Group (ECOG) 0 or 1 Exclusion Criteria: • More than one prior anti - tubulin / microtubule agent • Corneal epitheliopathy or any eye disorder that may predispose the patients to this condition • Symptomatic Central nervous system (CNS) metastases and / or carcinomatous meningitis • Contraindication to both CT and MRI contrast agents • Active hepatitis B or C infection • Pregnant or breast - feeding patients • Tumor type specific exclusion criteria

In filamentous cells of the bacterium Rhodobacter sphaeroides, the tubulin homolog FtsZ (tagged with CFP) forms dot - like and spiral structures in two distinct populationIn filamentous cells of the bacterium Rhodobacter sphaeroides, the tubulin homolog FtsZ (tagged with CFP) forms dot - like and spiral structures in two distinct populationin two distinct populations.

The image shows a midbody purified from HeLa cells synchronized in cytokinesis that has been fixed and immuno - stained to detect tubulin (shown in red) and Citron kinase (shown in green).

Now Roll - Mecak is applying her mathematical mind to cell biology in an effort to crack the tubulin code.

E — G, In contrast, exercise increased hippocampal BDNF levels across all three genotypes (E, ANOVA effect of exercise, p = 0.035), with no change in α - tubulin as a loading control (FIn contrast, exercise increased hippocampal BDNF levels across all three genotypes (E, ANOVA effect of exercise, p = 0.035), with no change in α - tubulin as a loading control (Fin α - tubulin as a loading control (F).

Neuronal outgrowths in 24 - well tissue culture dishes were fixed, permeabilized and blocked as above prior to staining with anti-β-III tubulin, 1 ∶ 200 (Chemicon mab1637 followed by secondary labeling with AF488 goat anti-mouse IgG1 (A21121, Invitrogen).

(A — D) Expression of β - III tubulin (A, red) and TH (B, green) in NSCs that had been differentiated for 4 weeks (C = nuclei, blue, D = merge).

389/12: 00 WD - repeat 47 is essential for the normal brain development through interaction with SCG10 in tubulin - associated processes.

This work examines how the carboxy - terminal tail domain of β - tubulin governs microtubule dynamic instability and the structure of plus ends using complementary in vivo and in vitro experiments.

Unfortunately, in neurons exposed to toxicants such as the mercury in thimerosal, the structural building blocks of the axon (molecules called tubulin) are disassembled, causing the axon to disintegrate.