Sentences with phrase «u of penicillin»

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2.5 million CD4 + T cells were seeded at a density of 0.8 × 106 cells / ml in RPMI containing 10 % fetal calf serum, 1 % penicillin / streptomycin, and 100 U / ml interleukin - 2 (IL - 2).
For induction and culture of mouse iPS cells, HFF cells were passaged using trypsin and cultured in medium containing DMEM, 10 % FBS (Hyclone), 1 % NEAA (Invitrogen), 2 mM L - glutamine (Invitrogen), 100 U / ml penicillin and 100 µg / ml streptomycin.
Cells were cultured at 37 °C in an atmosphere of 5 % CO2 in Rosewell Park Memorial Institute (RPMI) 1640 medium (Mediatech, Herndon, VA, USA) containing 10 % fetal bovine serum (FBS)(Gemini Bio-Products, Woodland, CA, USA), 100 U / ml penicillin / streptomycin and 2 mM glutamine (Invitrogen, Carlsbad, CA, USA).
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