Not exact matches
2.5 million CD4 + T cells were seeded at a density
of 0.8 × 106 cells / ml in RPMI containing 10 % fetal calf serum, 1 %
penicillin / streptomycin, and 100
U / ml interleukin - 2 (IL - 2).
For induction and culture
of mouse iPS cells, HFF cells were passaged using trypsin and cultured in medium containing DMEM, 10 % FBS (Hyclone), 1 % NEAA (Invitrogen), 2 mM L - glutamine (Invitrogen), 100
U / ml
penicillin and 100 µg / ml streptomycin.
Cells were cultured at 37 °C in an atmosphere
of 5 % CO2 in Rosewell Park Memorial Institute (RPMI) 1640 medium (Mediatech, Herndon, VA, USA) containing 10 % fetal bovine serum (FBS)(Gemini Bio-Products, Woodland, CA, USA), 100
U / ml
penicillin / streptomycin and 2 mM glutamine (Invitrogen, Carlsbad, CA, USA).