Sentences with phrase «using breast cancer cell lines»

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To see whether cancer stem cell renewal involves a chain of events similar to that used by embryonic stem cells, and whether the process was affected by oxygen levels, Semenza and graduate student Chuanzhao Zhang focused their studies on two human breast cancer cell lines that responded to low oxygen by ramping up production of the protein ALKBH5, which removes methyl groups from mRNAs.

Cheng, an assistant professor of medicine in hematology / oncology at Feinberg, provided the cell lines and NanoFlare targets the researchers used to model blood samples taken from breast cancer patients.

In the lab, the scientific team used an approach that combined functional RNAi analysis with gene expression analysis in breast cancer - derived cell lines and in human breast cancers replicated in mice.

Using a new TaqMan PCR - based technique, the researchers screened a number of cancer cell lines from various tissues, and discovered that tRNA halves were specifically expressed in large quantities in sex hormone - dependent cancers, i.e., estrogen receptor (ER)- positive breast cancer and androgen receptor (AR)- positive prostate cancer that are driven by the hormones estrogen and testosterone.

al. 47, the expression profiling of ABC transporters in a drug - resistant breast cancer cell line was investigated using AmpArray.

Using a qRT - PCR - based method, we found dramatic decreases of epithelial miRNAs (miR200c and miR205) in the F3 subpopulations compared with the parental breast cancer cell lines (Figure 6e).

Using cutting - edge techniques enabled by next - generation sequencing, the authors generated complete methylome maps at single nucleotide resolution in a low - passage breast cancer cell line and normal breast tissue (primary human mammary epithelial cells).

Conditions for hypoxia / reoxygenation cycles were optimized using two human metastatic breast cancer cell lines (MDA - MB 231 and BCM2).

Although the differing effects of hypoxia reported here are in conflict with previously published data, where no contrasting effect was seen (18 — 20), our data was produced using 13 primary breast cancer samples, as well as established cell lines, and the opposing effects in hormone receptor — positive and negative tumors, measured using multiple in vitro and in vivo CSC assays, were consistent in all cases.