Sentences with phrase «using gene mapping»

Using gene mapping and gene validation, researchers from Boston University School of Medicine (BUSM) were able to identify cytoplasmic FMR1 - interacting protein 2 (CYFIP2) as a major genetic risk factor for binge eating.

Using gene mapping techniques, the researchers zeroed in on a mutation in the gene for hypocretin receptor 2.

Not exact matches

The markers have also been used to produce a detailed map of the sugarcane genome that shows how the genes are linked to each other.

In this month's issue of Genome Research, Elizabeth Stewart and her colleagues at Stanford University present this new map, which places about 8000 landmarks along the genome's 3 billion bases — DNA's building blocks — yielding twice the resolution of gene maps currently in use.

To test that idea in the ragworm, Tomer used a technique he had developed to examine the complex brains of small creatures with unprecedented clarity: He created a high - resolution map of the worm's brain cells according to the genes they express, not just their shape and location.

But the genetic factors underpinning seed coat permeability remained a mystery until Ma and his team used a map - based cloning approach to hone in on GmHs1 - 1 as the gene responsible for hard seededness.

To learn more, Zanders and her colleagues used genetic mapping to reveal the complex landscape of multiple meiotic drive genes on chromosome 3 of S. kambucha.

Genes and words have several similarities, and language evolution has conventionally been mapped using a «family tree» format.

Genetic tools still under construction, such as a haplotype map that will be used to discern genetic variation behind common diseases, could further accelerate the search for disease genes.

With chronically infected mice as their model, the researchers used a new technology called ATAC - seq to map the regulatory regions of the genome — the sections of DNA involved in switching genes on and off — in the animals» exhausted and functional CD8 + T cells.

The new research focused on just nine genes, those most strongly associated with autism in recent sequencing studies, and investigated their effects using precise maps of gene expression during human brain development.

The vectors are customizable and could potentially be used as part of a gene therapy to treat neurodegenerative disorders that affect the entire central nervous system, such as Huntington's disease, or to help map or modulate neuronal circuits and understand how they change during disease.

By using random series of 30 nucleotides — the building blocks of RNA and DNA — he thought he could give neurons unique barcodes, linking the barcodes across synapses, then create maps of their connections using high - throughput gene sequencing technology.

«For our new systemic viral vectors — AAV PHP.S and AAV PHP.eB — there are many potential uses, from mapping circuits in the periphery and fast screening of gene regulatory elements to genome editing with powerful tools such as CRISPR - Cas9,» says Gradinaru.

The goal was to make fragments that could be used to map genes and sequence the genome.

By mapping the neural processes involved as birds learn mating songs, scientists hope to someday use that knowledge to target specific genes disrupting speech in patients with autism or other neurodevelopmental conditions.

We further assessed ILS using insertions and deletions (indels)(69), because they have less homoplasy (convergence) than single nucleotides (SM9), and unlike gene trees, indels can be examined as discrete characters mapped to a reference tree without the added inference of constructing trees from them.

Using bioinformatics tools to identify and map out specific components and regulatory interconnections, the study team found highly dynamic activities during CD8 + T cell responses: a distinct repertoire of super enhancers — groups of enhancers that interact with promoters to drive gene transcription, new groups of enhancers that jump into activity only in the memory cell stage, and extensive re-wiring of regulatory circuits from one cell stage to another.

Using a technique called whole - genome random sequencing, a TIGR team mapped the entire two - million - gene sequence of Thermotoga maritima, a bacterium first plucked from hot geothermal springs in Vulcano, Italy.

Positional cloning is a genetic mapping technique used to pinpoint the location of specific traits of interest, such as disease - causing genes or mutations, within the genome.

That map contains several so - called islands that are unique to A. borkumensis, such as a set of genes that allow the organism to break down the alkanes in oil and use them as food.

The map will help to hunt down disease - causing genes, which may be used to design new drugs, diagnose illness, and help vulnerable patients avoid bad drug reactions.

Pairwise alignments were calculated using Shuffle - LAGAN (window size, 400 bp; step size 40 bp; translated anchoring), a glocal alignment algorithm that is able to calculate optimal alignments by using both local alignments and global maps of sequence rearrangements (e.g. duplications of the fiber gene in adenovirus genomes with 2 fibers)[57].

For example, many studies on human microbiota identify species (or operational taxonomic units) and map evolutionary relationships using the 16S ribosomal RNA gene.

When mapped to the genome, they tell us not only about the level of expression for various genes, but also which transcription start sites were used.

Approach: Wu's group used Brainbow, a transgenic technique originally devised to map individual neurons in the brain, to label cells stochastically using gene cassettes that code for four uniquely expressed fluorescent proteins.

The atlas now also includes RNA transcript data for 27 of these organ - specific tissues using next generation sequencing, providing a tissue distribution map of both protein and gene expression.

In the present study, to elucidate the neural mechanism underlying this behavior, we used an immediate early gene (IEG) to map the active brain regions of Japanese honeybee workers during the formation of a defensive bee ball.

Genome mapping is used to identify and record the location of genes and the distances between genes on a chromosome.

Their study used a pioneering genetic technique to analyse maps of DNA regions linked to an inherited risk of breast cancer and identify the actual genes involved in raising a woman's risk.

They used yeast cells to model the disease, which allowed them to look at over 5,000 genes to map out which ones caused more or fewer cells to die.

The new dependency data complement the Dependency Map team's ongoing efforts to use functional genomic technologies like CRISPR and RNA interference (RNAi) to locate vulnerabilities that arise within cancer cells as they compensate for the loss of critical genes due to mutations or expression changes.

Reads were mapped to the S. viridis A10.1 reference genome (phytozome.jgi.doe.gov; v1.1) using TopHat2 (v2.1.0) and an a priori set of 35,214 gene models.

And to do this, she used the latest technology to sequence the DNA — map its building blocks to read its genes.

Global analysis of candidate genes important for fitness in a competitive biofilm using DNA - array - based transposon mapping.

RNA - seq reads from wild - type and mutant libraries were mapped to the S. viridis reference genome (v1) and annotated gene models used to quantify transcript abundances (Supplemental Data Set 3).

The RNA being used to map genes in the genome has been obtained from another of Sally's progeny obtained in 2015.

«Use of peptide tagging to detect proteins expressed from cloned genes: deletion mapping functional domains of Drosophila hsp 70.»

The study, «A gene - based association method for mapping traits using reference transcriptome data,» was funded by the National Institutes of Health.

Their contributions to the yeast community include physical mapping methods, synthetic lethality screen approaches for identifying cross-species candidate genes as potential cancer drug targets, and a widely used set of vectors and yeast host strains that have been instrumental in work that has led to countless discoveries in recent decades.

To capture additional spliced reads that remained unmapped, we remapped all unmapped reads against the OGSv2 predicted transcriptome using the — very - sensitive - local option in bowtie2 [47], and added the counts of reads mapped to each gene to the counts derived from the genome mapping.

This section invites manuscripts describing (a) Linkage, association, substitution or positional mapping and epigenetic studies in any species; (b) Validation studies of candidate genes using genetically - engineered mutant model organisms; (c) Studies focused on epistatis and gene - environment interactions; (d) Analysis of the functional implications of genomic sequence variation and aim to attach physiological or pharmacogenomic relevance to alterations in genes or proteins; (e) Studies of DNA copy number variants, non-coding RNA, genome deletions, insertions, duplications and other single nucleotide polymorphisms and their relevance to physiology or pharmacology in humans or model organisms, in vitro or in vivo; and (f) Theoretical approaches to analysis of sequence variation.

We then compiled a list of 881 predicted mRNA targets that unambiguously mapped to unique Entrez Gene IDs, and performed a number of enrichment analyses using the WebGestalt platform [21] to identify functional categories and pathways overrepresented in this gene lGene IDs, and performed a number of enrichment analyses using the WebGestalt platform [21] to identify functional categories and pathways overrepresented in this gene lgene list.

With funding for over a decade from the NSF and the USDA, the Rice Diversity Project supports QTL mapping, Genome Wide Association Studies (GWAS), and gene discovery using a suite of open - source genetic, genomic and bioinformatic resources developed in the McCouch lab.

Using genetic maps, scientists know that the mutated gene responsible for this tolerance lies on chromosome 1, now they just need to find it.

Genes affecting autoimmune type 1 diabetes susceptibility in the nonobese diabetic (NOD) mouse (Idd loci) have been mapped using a congenic strain breeding strategy.

«The Department of Health and its Gene Therapy Advisory Committee are currently working with other regulators in the field to produce a «Regulatory Route Map» to provide further clarity on the regulatory requirements for the clinical use of stem cells.»

The GENSAT project aims to map the expression of genes in the central nervous system of the mouse, using in situ hybridization and transgenic mouse techniques.

Mapping the expression profiles of all genes one cell at a time revealed unexpected heterogeneity in the stem cell - derived lung cells, and the research team at the CReM used this information to improve the airway cells engineered in the lab.

The search engine recognizes common gene identifiers; the conversion to Affymetrix probe set IDs is done using Ensembl BioMart [77] mappings.