Human pluripotent stem
cells (hPSCs), including human embryonic stem
cells (hESCs) and human induced pluripotent stem
cells (hiPSCs), are known to be vulnerable to apoptosis upon
various technical
manipulation, such as single
cell dissociation, freezing and thawing, etc., which hinder their use for clonal isolation in gene transfer, differentiation and FACS
cell sorting.
Our studies may also serve to enhance competence for somatic embryogenesis in recalcitrant genotypes / explants through the
manipulation of overall chromatin organisation in target
cells using
various chromatin - related drugs.