Sentences with phrase «vero cells»

The virus was re-isolated from the affected ducks and replicated well in primary duck embryo fibroblasts and Vero cells, causing the cytopathic effect.

The virus also replicated in Vero cells, and focal CPE appeared with the cells rounding up and floating free from the surface of the flask 60 hrs after infection (Fig. 4C and D).

Viral titers were measured using plaque assay with Vero cells as previously described (12).

Viral stocks were titrated on Vero cells (African Green Monkey Kidney) supplemented with 10 % fetal bovine serum (FBS) as well as penicillin - streptomycin (1 %) and fungizone (0,05 %).

By integrating the sequence data and the associated metadata, we have established laboratory and bioinformatics - based methods that enable us to investigate scientific questions including: the tropism of ZIKV for dendritic cells and the antiviral response of the cells during infection (Bowen et al. 2017); investigating the phylogenetic diversity of strains obtained by Biodefense and Emerging Infections (BEI) Resources and distributed to virologists (manuscript in preparation); the genetic determinants of ZIKV host adaptation in C6 / 36 mosquito vs. Vero cells; and the diversity of ZIKV circulating in limited geographic regions, including Barbados, Colombia, and the state of Chiapas, Mexico.

«We tried to isolate the virus in two cell lines, the standard Vero cells, which are mammalian in origin, and in C6 / 36 cells, which are derived from mosquitoes,» he explained.

Vero cells are a particular kind of kidney monkey cells.

HE - stained, non-infected DEF control (A); HE stained infected cells, 52 hrs post-infection, showing cell disruption with a large number of red - stained particles (B); Non-infected Vero cell monolayer (C); Infected Vero cells rounded up and focal detachment 60 hrs post-infection (D); Thin section of the infected cell, 24 hrs post-infection, showing dense particles (white arrow head) and virions (arrow) within the vesicles in the cytoplasm (N, nucleus).

Not exact matches

Many researchers use «Vero» cells as these typically allow Zika virus to replicate at high efficiency, but that may not be the best choice for semen samples, said Atkinson.

Vero and C6 / 36 cell cultures were maintained at 37 °C and 28 °C, respectively, and were examined daily for 10 days for evidence of viral cytopathic effects (CPE).

The other FDA - approved assay, the «DNA Fluorescence Method,» involves incubating an indicator cell line called Vero with test samples, waiting three to five days, and staining with Hoechst DNA stain.

The homogenates were centrifuged and SARS - CoV titers in the clarified fluids were determined by serial dilution in quadruplicate wells of Vero E6 cells in 96 - well plates.

0.1 mL of supernatant was added to BHK - 21 and African green monkey kidney (Vero) cell cultures (ATCC, Manassas, VA) and monitored as above.

Vero E6 cells without virus inoculation and HeLa cells without ACE2 were used as negative control.