Sentences with phrase «wt animals»

Importantly, none of these Abs bound to native mouse AS in WT animals, consistent with their intended selectivity for human - derived AS species.

(WT animals were free of recalcitrant aggregate).

Furthermore, whereas XpdTTD / TTD mice developed kyphosis earlier than wt animals (onset ~ 3 mo versus 12 — 20 mo), compound heterozygous XpdTTD / † XPCS mice did not (Figure 3B).

In WT animals, responses to all tastants except 5 mm quinine were larger under urethane anesthesia than with pentobarbital (Fig. 7B, Table 4), but this difference in response magnitude with anesthetic did not occur in the KO mice (Fig. 7C, Table 4), with the exception of sucrose.

To rule out that the staining was coming from the aggregates present in the inoculum, cultured islets from WT animals, which do not express hIAPP, were incubated with the same amount of old Tg islet homogenate.

Individual differences between Tg / Tg and Tg / WT animals were studied by the Bonferroni posttest.

Not exact matches

(E and F) Black bars represent the animals inoculated with old Tg - hIAPP pancreas homogenate, and white bars correspond to the controls injected with WT pancreas.

(A and B) Figures correspond to representative pictures of the five animals studied, except in the case of the animals treated with WT pancreas homogenate and sacrificed at 10 wk old; in which, 2 animals died of unrelated reasons.

The percentage of hyperglycemic animals increased progressively with time after inoculation of Tg pancreas extracts, whereas up to 20 wk of age, no animals of the Tg / WT group reached the threshold to be considered hyperglycemic.

Fasting blood glucose level became significantly higher in animals injected with Tg - hIAPP pancreas homogenate containing IAPP aggregates, as early as 8 wk compared with mice treated with WT pancreas extracts (Fig. 4 A).

Nonetheless, responses in KO animals to most stimuli (100 mm NH4Cl, 500 mm sucrose, 300 mm MSG with amiloride, 5 — 20 mm citric acid, and 5 — 20 mm HCl) were reduced compared with WT (Fig. 6B, Table 4), suggesting a role for 5 - HT3 signaling in more than just acid and salt transmission; that is, tastants likely to evoke direct type III cell - mediated signaling.

Normalized responses of Fura -2-AM loaded geniculate ganglion neurons revealed that a subset (solid circles; 5/19) of WT neurons were activated by 10 μm 5 - HT; no neurons from 5 - HT3AKO animals (open circles; 0/25) responded to 5 - HT, yet all responded to 10 μm ATP and 55 mm KCl.

To induce diabetes in WT mice, animals were treated with STZ, as previously described (Wilson and Leiter, 1990).

Notably, XpdTTD / † XPCS animals had a partial TTD hair phenotype, correlating with low levels of Xpd † XPCS expression, whereas XpdTTD / XPCS animals had wt hair, correlating with normal expression levels from the viable XpdXPCS allele (Table 2 and unpublished data).

Primers 8134 and 8135 efficiently amplify full length fragment in wild type animals (WT) while mutant animals show homozygous deletion in W01A8.1 gene.

mRNA expression from the targeted allele could be detected in embryonic stem cells by RT - PCR (Figure 1D), although expression was reduced approximately 5-fold relative to wt mRNA transcript levels as determined by Northern blotting of RNA from the testis of heterozygous animals (Figure 1E).

If depicted as relative value, the single values per mouse were normalized to the average of the littermate WT or control treated animals.

Curcumin dissolved in corn oil was fed orally to both 6 and 24 - month - old animals at a dose of 30 mg ml - 1 kg - 1 body wt daily for one month (Reddy and Lokesh 1996).

Similarly, iNOS expression levels were elevated in vehicle - treated PDGF - AS mice, but held to WT levels in animals receiving AFF 1 vaccine.

[19] In the current study, [15] PDGF - AS mice exhibited an increased number and intensity of areas staining for reactive microglia (using Iba1) and astroglia (using GFAP), but vaccination with AFF 1 prevented nearly all of this disease - associated excess, with treated animals exhibiting only the burden of reactive glia present in similar - aged WT mice.

Remarkably, treatment with 1H7, 9E4, or 5C1 were each able to completely or near - completely prevent the emergence of these deficits, with treated animals performing at levels not significantly different from those of their WT controls.

Animals were administered 10 mg / kg of candidate mAbs weekly for 6 mo; control animals (WT and TG) were immunized with IgG1 as a contAnimals were administered 10 mg / kg of candidate mAbs weekly for 6 mo; control animals (WT and TG) were immunized with IgG1 as a contanimals (WT and TG) were immunized with IgG1 as a control Ab.

mThy1 - AS controls were unable to recruit even one hindlimb to prevent their own falling, but AFF 1 treatment maintained such engagement to half of WT control animals» levels (average ≈ 1.0 vs. ≈ 1.75 hindlimbs engaged — all Figure 2).

The exponential formula (RER = 70 [body wt in kg0.75]-RRB- can be used for animals of any body weight, whereas the linear formula (RER = 30 × [body wt in kg] + 70) is restricted for use in animals that weigh > 2 kg and < 45 kg.