Sentences with phrase «wt expressing cells»
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To examine this, we transiently expressed CXCR4Δ18 or wt CXCR4 as a control in 293T cells, which have low endogenous CXCR4 expression.
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Further, consistent with T cell phenotypes in nontumor - bearing mice (Fig. 2), we observed an increased percentage of spleen - derived CD8 + T cells expressing high levels of the activation marker CD44 in DGKζ − / − or DKO mice that had been inoculated with tumor when compared with Cbl - b − / − or WT mice (Fig. 4C).
Expression of the familial PD - linked A53T or A30P mutant ASs in cell models results in an increased proportion of CTTAS being produced than is the case when expressing the WT protein; consistent with this, a higher proportion of the total soluble AS in human brain tissue with AS neuropathology is CTTAS, and a higher proportion yet of the total AS in insoluble AS deposits.
(A) In wild - type (wt; N2), UNC - 9:: GFP expressed in B motor neurons (Pacr - 5:: unc - 9:: gfp) visualizes puncta in the ventral nerve cord localized near B cell bodies.
(F and G) The levels of LARP7 protein (F) and mRNA (G) as well as 7SK snRNA level (G) in control, shLARP7 - 2 cells and rescue cells (expressing an shRNA - resistant WT LARP7 cDNA) were examined by Western blotting and qRT - PCR, respectively.
After eGFP - expressing CD4 + T cells and WT counterparts were treated with vehicle and indicated inhibitors, respectively, they were mixed with the collagen gel and monitored for cell migration.
(B) Ratio of LPA receptor - deficient (Lpar2 - / -, Lpar5 - / - or Lpar6 - / --RRB- cells to eGFP - expressing (WT) cells that migrated into the spleen, peripheral LN, and MLN after adoptive transfer into WT recipient mice.
While this approach did not allow us to examine the impact of selective EBI2 - deficiency in DCs on WT B - lineage cells, the experiment did allow assessment of the response of EBI2 - deficient B cells in mice that lack EBI2 on CD11c + DCs while retaining EBI2 on approximately half of all non-CD11c expressing cells (such as helper T cells).