Sentences with phrase «wt geniculate»
Approximately 25 % of all WT geniculate neurons (5/19; Fig. 5) responded to exogenous 5 - HT with an increase in intracellular calcium, whereas no neurons from 5 - HT3AKO mice responded (0/25, p = 0.011, Fisher's exact test; Fig. 5).
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A, RT - PCR showing the presence of 5 - HT3A transcripts in brain and geniculate ganglion of WT mice, but the lack of 5 - HT3A mRNA in geniculate ganglion of 5 - HT3AKO mice.
RNA was extracted from geniculate ganglia of 5 - HT3AKO and WT mice (3 mice each) according to manufacturer's instructions using the RNeasy Micro kit (Qiagen), including a 30 min DNase I treatment at room temperature for removal of genomic DNA.
Normalized responses of Fura -2-AM loaded geniculate ganglion neurons revealed that a subset (solid circles; 5/19) of WT neurons were activated by 10 μm 5 - HT; no neurons from 5 - HT3AKO animals (open circles; 0/25) responded to 5 - HT, yet all responded to 10 μm ATP and 55 mm KCl.
In addition, we tested whether other 5 - HT receptors were upregulated after genetic deletion of 5 - HT3 by measuring 5 - HT responses in isolated geniculate ganglion neurons of WT and 5 - HT3AKO mice.