Sentences with phrase «western blot analysis»

If resources allow, cats testing positive by the ELISA test should be retested by sending the appropriate sample to a laboratory for IFA or western blot analysis.

Western blot analysis of of extracts from cells expressing different activated tyrosine kinase proteins, using Phospho - PDGF Receptor (Tyr754)(23B2) Rabbit.

(E) Western blot analysis showing that overexpression of PSD - 95 restores BDNF signaling in Ube3A RNAi knockdown SH - SY5Y cells.

The proteins were transferred from the gels onto Immobilon P membrane (Millipore Corp.) at 200 mA for 2 hours for Western blot analysis.

(D) Western blot analysis showing that overexpression of PSD - 95 enhances BDNF - induced p - Akt and p - CaMKII, but not p - Erk or p - TrkB in SH - SY5Y - TrkB transfected cells.

(E) Luciferase assay and western blot analysis of 3T3 cells following transfection with PORE reporter and overnight treatment with indicated cAMP analogues.

Western blot analysis of UNC - 7 isoforms translated in rabbit reticulocytes.

The researchers used Western Blot analysis to confirm the proteomic results.

ER - α was transiently overexpressed in 231 and 468 cells using the V16 - ERα plasmid (Addgene # 11351) and expression was confirmed by Western blot analysis (Fig. 4A).

(D) Differentiated Ara - C treated P19 cells were treated with indicated doses of proteasome inhibitors, followed by western blot analysis of Oct4 protein expression.

(F) Undifferentiated P19 cells, untreated or treated with 10 uM MG - 132 for 1 hour, followed by western blot analysis.

A, representative Western blot analysis measuring expression of V16 - ER - α plasmid; top band, V16 - ER - α; bottom band, endogenous ER - α.

(E) Western blot analysis of iPS - RPE protein expression using antibodies against a panel of RPE cell markers.

(A) 106 BaF3 Aut (V658I), Aut (F958V) and BaF3 cells stably transduced with M - RAS or (B) JAK1 V658I and F958V mutants were treated for 30 min with 500 nM CMP6 inhibitor or with 0.1 % DMSO as a control (− condition), lysed and subjected to Western blot analysis.

(B) BaF3 cells stably transduced with V617F, Y931C or V617F / Y931C mutant were lysed and subjected to Western blot analysis.

We chose one representative JAK1 mutation - positive autonomous BaF3 clone for each of the 25 JAK1 mutations and performed Western blot analysis to assess the activation status of JAK1, STAT5 and ERK1 / 2.

(E) 106 BaF3 cells stably transduced with JAK2 V617F, Y931C and V617F / Y931C double JAK2 mutant were treated for 30 min with 300 nM CMP6 inhibitor or with DMSO as a control (− condition), lysed and subjected to Western blot analysis.

Western blot analysis showed that the COX - 2 protein levels were significantly decreased in the STAT6 DN cells compared to the parental or vector - containing cells (Figure 2a).

Western blot analysis indicated that the complemented clone ospC7 / ospC +4 synthesized OspC at a level comparable to the WT parental strain and was influenced by pH, as described in ref.

By Western blot analysis, we first examined whether the Janus - activated kinase (JAK)- STAT pathway — activated ISGF3 complex was involved in IFNα - induced RIG - G up - regulation.

For Western blot analysis, 106 BaF3 cells were starved for 4 h and lysed in 250 μL of Laemmli buffer (BioRad).

Western blot analysis of whole - cell lysates demonstrated that ospC7 no longer synthesized OspC (Fig. 3).

Anti-goat MSTN monoclonal antibody (1 ∶ 2000 dilution) and 1 ∶ 1000 dilution of a mouse anti-actin antibody (Sigma, A4700) were used for the western blot analysis.

Western blot analysis showed that phosphoinositide - dependent protein kinase 1, the upstream kinase that phosphorylates Akt, and several phosphatases (phosphatase and tensin homolog deleted on chromosome 10, protein phosphatase 1, and protein phosphatase 2A), known as negative regulators of the PI3K / Akt signaling pathway, were unchanged upon VPA treatment (Supplemental Fig. 4E), indicating that VPA might act on Akt directly.

Using a 2 - mm micropunch (Harris Uni-core; EMS, Hatfield, Pennsylvania), tissue was collected from 2 major components of the medullary 5 - HT system, the raphé obscurus and paragigantocellularis lateralis (PGCL), according to the atlas of Paxinos and Huang, 19 and standardized protein samples were obtained for Western blot analysis in each SIDS case and control.20 Twenty - micrometer tissue sections were collected from the remaining blocks in a standardized manner for tissue receptor autoradiography.

Western blot analysis of antibody specificity has been done using a routine sample setup composed of IgG / HSA - depleted human plasma and protein lysates from a limited number of human tissues and cell lines.

Western blot analysis of chondroitin sulfate proteoglycans and Olig2 was performed as previously described [57], using anti-phosphacan monoclonal (1 ∶ 1000, 3F8, Developmental Studies Hybridoma Bank), anti-CSPG4 / NG2 monoclonal (1 ∶ 2000, Chemicon), anti-Olig2 polyclonal (1 ∶ 4000, Chemicon) and anti-b-tubulin monoclonal antibody (1 ∶ 1000, Santa Cruz).

Western Blot analysis of LGALS3 expression in Hela S3 NE.

For tau deposition, Braak stage was IV / VI, and the Western blot analysis score was 9c / 10.

Western Blot analysis of LGALS3 expression in mouse brain.

Western Blot analysis of LGALS3 expression in human stomach.

For an ILP2 western blot analysis, we extracted hemolymph from 24 h AL3E larvae.

Western blot analysis revealed high levels of p - mTOR associated with high levels of p - p70S6K, p - rpS6, p - 4E - BP1, and total eIF4E in both cell lines (Fig. 1).

Expression of adeno - myrAkt in infected cells was confirmed by Western blot analysis using a monoclonal antibody specific for the HA tag.

Western blot analysis was done using standard methods as described previously (21).

Western blot analysis confirmed adequate inhibition of protein expression in transiently transfected cells.

A, Western blot analysis after subcellular fractionation showed that Karpas 299 and SU - DHL1 cells express phosphorylated mTOR, 4E - BP1, and rpS6 as well as total eIF4E predominantly in the cytoplasm.

Western blot analysis showed almost complete silencing of AKT1 gene product that was associated with decreased levels of p - mTOR (C).

Western blot analysis confirmed that this was indeed the case.

SN designed and performed the Western blot analysis and helped to design and perform tumor - sphere formation assays.

Additionally, Western blot analysis indicated that the expression level of p21waf1 / cip1 gene was also enhanced by the ectopic expression of DDX3 in HuH - 7, HeLa, and 293T cells (Fig. 2B).

Expression of adeno - myrAkt in infected cells was confirmed by Western blot analysis using the anti-HA antibody.

Western blot analysis of GAPDH in various tissues and cell lines using Proteintech antibody 60004 -1-Ig at a dilution of 1:10000.

By Western blot analysis using rat brain extracts of cortex, hypothalamus, midbrain, and hindbrain, the antibody specifically labels a single band.

Data was kindly provided by Ann Hammarstedt (Department of Molecular and Clinical Medicine, Gothenburg University, Sweden), who performed the Western blot analysis of Akt proteins, incubation of cells in MCD - media, and subsequent qRT - PCR analysis.

(i, k) Western blot analysis of AKT phosphorylation (Ser473) in control (shCtr) and Tie2 - silenced (shTie2 I / shTie2 II) BP (i) and HUVEC (k) upon stimulation with rhAng1 or rhAng2.

(f) Western blot analysis of phosphorylated FOXO3A (pFOXO3A) and total FOXO3A in BP stimulated with rhAng1 or rhAng2 for 30 min.

Western blot analysis of Beta - actin in various tissues and cell lines using Proteintech antibody 60008 -1-Ig at a dilution of 1:5000.

After three rounds, the efficiency of depletion was tested by Western blot analysis using C - terminal (23 — 37)-- specific anti — hIAPP antibody.

These results were also confirmed by Western blot analysis (Fig. 2C).