This has implications for the use of
deep sequencing analysis of immunization studies: if the
sequence of J3 had been used as a reference to filter the
sequences from llama 9 (
without prior screening of VHH from llama 9), no J3 - like antibodies would have been found, 3E3 would not have been identified and it would have falsely appeared that the immunization of llama 9 had failed.