Using the gene - editing tool CRISPR - Cas9 to turn off certain
genes in a mouse zygote as well as other new techniques to enrich the pluripotent stem cells of a rat, the group managed to grow various rat organs (a pancreas, heart, and eyes) in a mouse embryo.
Modifying a histone called H3K27 shuts down the activity of some imprinted
genes in mice, Howard Hughes Medical Institute (HHMI) Investigator Yi Zhang and colleagues report July 19 in the journal Nature.
This study led to the discovery of 18 new imprinted genes, validated some known genes and resolved the disputed status of some others to provide a gold standard list of 93 imprinted
genes in mouse.
After four weeks of such training, researchers reactivated the p25
gene in the mice for six weeks and then tested them to see if they recalled their shocking experiences.
These experiments revealed that the CLOCK protein targets the per
gene in mice and both the per and tim genes in flies.
To probe this gene's role in brain development, Greenough's collaborators at the University of Amsterdam and at Erasmus University in Rotterdam, the Netherlands, knocked out
the gene in mice.
The team used CRISPR to fix a blindness - causing
gene in mice.
The researchers used the dead guide RNAs to turn on the Pdx
gene in the mice's livers, which caused the liver cells to produce insulin, reversing the mice's diabetes.
Northwestern Medicine scientists have identified a small RNA molecule called miR - 182 that can suppress cancer - causing
genes in mice with glioblastoma mulitforme (GBM), a deadly and incurable type of brain tumor.
Initial testing of mTOR inhibition was achieved by direct deletion of the mTOR
gene in mice.
Molecular geneticist Cheng Chi Lee, developmental biologist Gregor Eichele, and their co-workers at the Baylor College of Medicine in Houston have isolated
a gene in mice and humans that shares 44 % of the amino acid sequence of the period (per) gene of the fruit fly Drosophila melanogaster.
The loss of a single
gene in mice can affect social behavior and impair their brains» ability to filter out distractions — both characteristics of several neurological diseases in humans.
«But there are 50 - plus MHC
genes in mice, and we don't know what most of them do.
Scientists have known for 20 years that SMN is necessary in every cell of the body, since disrupting
the gene in a mouse causes early embryonic death, before muscle or nerve cells form.
To find answers to these questions, the scientists studied the gene regulation of the DPP4
gene in mice that are prone to obesity.
In earlier studies, Wang and his colleagues had revealed that
a gene in mice called Pad4 (peptidylarginine deiminase 4) produces an enzyme that plays an important role in protecting the body from infection.
However, cancer cells may instead be coaxed to turn back into normal tissue simply by reactivating a single gene, according to a study that found that restoring normal levels of a human colorectal cancer
gene in mice stopped tumor growth and re-established normal intestinal function within only 4 days.
By knocking out individual
genes in the mouse, they can systematically discover any that have a crucial biological role, or are linked with disease.
In a mouse model of eczema, loss of the HTR7
gene in mice led to significantly less scratching and less severe skin lesions.
The European Mouse Disease Clinic (EUMODIC) brought together scientists from across Europe to investigate the functions of 320
genes in mice.
The researchers therefore decided to take a closer look at the protein - encoding
genes in the mouse tumors.
Physiologist Tejvir Khurana at the University of Pennsylvania has discovered
a gene in mice that allows them to run about three and a half miles on an exercise wheel — more than the equivalent of a mouse marathon — without fatigue.
Buxbaum and his coworkers point out that FOXP2 is also expressed in the brains of songbirds such as finches and canaries, and further studies of
the gene in mice might provide a better understanding of its role in human communication.
To try to determine how those changes influenced the gene's function, that group put the human version of
the gene in mice.
A few weeks later, in one of those odd coincidences of medical science, molecular geneticist Masashi Yanagisawa and his colleagues at the Howard Hughes Medical Institute and the University of Texas Southwestern Medical Center announced they had found a closely associated
gene in mice.
When Yanagisawa found the sleep
gene in mice, he was studying a gene for eating.
A single gene appears to play a crucial role in coordinating the immune system and metabolism, and deleting
the gene in mice reduces body fat and extends lifespan, according to new research by scientists at the Jean Mayer USDA Human Nutrition Research Center (USDA HNRCA) on Aging at Tufts University and Yale University School of Medicine.
Deletion of the GM - CSF
gene in the mouse led to reduction and impaired regulatory function of gut tissue macrophages and dendritic cells which compromised induction of tolerance to food antigens and increased mice susceptibility to IBD.
The researchers used tools of epigenomic analysis to trace the specific epigenetic switches controlling each of thousands of
genes in both mouse and human retinal cells as the cells progressed through development.
«We anticipate a similar mode of action may operate in other organisms because similar RNAs have been found for clock
genes in mice.
For a decade, the group at North Carolina has studied a large family of
genes in mice called L1, some of which are dormant.
For their experiments, the researchers mutated one of the two copies of the KRAS
gene in mice.
Moving their studies from cells to whole animals, the researchers tested the effects of knocking out the Zbp1
gene in mice infected with influenza.
«Therefore, their genes operate very well at the early stages of their lives.There's no sense having
a gene in a mouse that's going to work in 10 years, because there's no way those genes will be passed on.»
But tinkering with
the gene in mice affects their communication too
Eliminating
genes in mice is a standard technique for determining their function; the resultant animals are called knockout mice.
We have identified some of
the genes in the mouse that are important for both learned fear and instinctive fear.
In addition, loss of the Esrp1
gene in mice leads to changes in the shape of the inner ear that is very similar to the situation with the siblings.
To find out why, they examined
genes in both mice and humans that turned on during peak brain development and spotted a DNA snippet, ARHGAP11B, that was active only in humans.
In an earlier study, Spector's team knocked out the Malat1
gene in mice and curiously, these mice had no apparent abnormalities, a fact that would seem improbable if Malat1 did in fact have an important function.
In previous research, Quaggin's lab showed that deleting
the gene in mouse models led to glaucoma, but the scientists didn't know how mutations impairing the gene affected humans.
Knocking out a single
gene in mice can produce symptoms of obsessive - compulsive disorder (OCD), researchers report in tomorrow's issue of Nature.
So whereas if you find a particular protein - coding gene in a human, you're going to find nearly the same
gene in a mouse most of the time, and that rule just doesn't work for regulatory elements.
To find out what these genes do, and which ones are master regulators of development, researchers have several approaches, including deactivating embryonic
genes in mice.
Researchers have just completed a library of all the active
genes in the mouse genome.
This image illustrates concepts in the press release titled «Infradian oscillation of circadian
genes in a mouse model of bipolar disorder».
When researchers deleted
the gene in mice with various liver injuries, they found that the livers replaced tissue mass quicker and showed reduced fibrosis in response to chemical injury.
To study these pathways, we use a range of in vitro and in vivo approaches, including manipulating
genes in mice and leveraging genome - wide analyses and chemical biology.
Sure enough, deleting the SIRT3
gene in mice abolished any of the protective properties of NR.
The function of the majority of
genes in the mouse and human genomes remains unknown.