Sentences with phrase «xt midgut»

A portion of the XT midgut contents were also placed in culture.

Standard PCR and RT - PCR for ospA, ospC and flaB were also performed with either SCID mouse tissues or XT midguts using primers described elsewhere [35].

Not exact matches

Given that our wild type B. burgdorferi acquired by xenodiagnostic ticks retain the ability to express ospC (also shown by RT - PCR in Fig 4), we can not rule out the possibility that a tick midgut - adapted phenotype, in the absence of salivation and feeding, contributed to the failure of the B. burgdorferi acquired from XT to infect SCID mice.

Panel A = positive control for IFA using B. burgdorferi culture; Panel B = XT from animal IH11 (treated); Panel C = XT from animal IK14 (treated); Panel D = XT from animal IL09 (treated); Panel E = positive control for DFA using midgut smear of tick that was capillary tube - fed B. burgdorferi; Panel F = XT from animal IP55 (untreated); Panel G = XT culture pellet from animal IP55 (untreated); Panel H = XT culture pellet from animal IK14 (treated).

In addition to staining the midgut contents with FITC - labeled polyclonal anti-Borrelia species antibody, we washed and re-stained this set and stained the second set of xenodiagnostic tick (XT) samples with an anti-OspA monoclonal antibody (CB10, obtained from J. Benach [41]-RRB-, followed by anti-mouse IgG - Alexa 488 (Molecular Probes).