Sentences with phrase «activated cell sorting»

CSC activity was assessed using well - established in vitro assays including mammosphere (25, 27) and clonogenic culture (28, 29) and fluorescence - activated cell sorting (FACS analysis of ALDH1 expression; ref.

Fluorescence activated cell sorting (FACS) analysis of HUF1 cells stained with the SSEA3 / 488 antibody complex, revealed a larger subpopulation of cells with little or no SSEA3 expression and a smaller subpopulation with detectable SSEA3 expression (Figure 2B).

Immunofluorescence analysis of the two populations, following overnight adherence to exclude analysis of non-viable cells, revealed that > 97 % of the SSEA3 - positive population expressed detectable SSEA3 / 488 fluorescence and 0 % of the SSEA3 - negative population expressed detectable SSEA3 / 488 fluorescence (Figure 2C), demonstrating that the fluorescence activated cell sorting process can purify viable subpopulations of cells from a heterogeneous somatic population.

Our research team recently expanded the utility of the transposon directed insertion sequencing (TraDIS) method by applying a physical separation of a transposon mutant library mediated by fluorescence activated cell sorting, rather than a fitness - based selection.

From other papers I would like to highlight the one by M. Mehrshad and enigmatic SAR202 cluster, the one by A. C. Lehours and biogeographic patterns of aerobic anoxygenic phototrophic bacteria and the one by E. M. Llufrio and how fluorescence activated cell sorting introduces oxidative stress and alters the metabolic state of cells.

Other often utilized technologies are fluorescence activated cell sorting, tissue culture, gene expression analysis, protein analysis, and lentiviral mediated gene transfer.

A total of 143 colonies of the HS237 line cultured in the Cell - IQ equipment was used in fluorescence - activated cell sorting (FACS) analysis.

Berkeley Lab's Len Pennacchio (left) and Axel Visel led the development of a new technique for identifying gene enhancers called SIF - seq, for site - specific integration fluorescence - activated cell sorting followed by sequencing.

300 μl of ice - cold 1 × binding buffer were then added to each sample and the samples were analyzed by fluorescence - activated cell sorting (FACS) within one hour for maximal signal.

GFP - positive cells, indicative of reactivated latent HIV - 1, were isolated by fluorescence activated cell sorting (FACS).

Our success in these areas is complemented by our substantial investment in cutting - edge facilities for nuclear magnetic resonance, protein crystallization, X-ray crystallography, transgenics, histochemistry, electron microscopy, confocal microscopy, and fluorescence activated cell sorting.

↵ 3 The abbreviations used are: HUVEC, human umbilical vein endothelial cell; DiI - Ac - LDL, 1,1 ′ - dioctadecyl - 3,3,3 ′, 3 ′ - tetramethyl - indocarbocyanine acetylated low - density lipoprotein; FACS, fluorescence - activated cell sorting; FGFR, fibroblast growth factor receptor; Flk, fetal liver kinase; ICAM, intercellular adhesion molecule; mAb, monoclonal antibody; PE, phycoerythrin; TNF, tumor necrosis factor; VCAM, vascular cell adhesion molecule.

Discovering that she could use a fluorescence - activated cell sorting machine to distinguish non-neuronal cells from neurons by making them glow in different colors was «a high point,» she says.

«It's the same process as for single cell genomics, but for aggregates of symbiotic bacteria and archaea,» said DOE JGI Microscale Applications Group head Rex Malmstrom of the technique called BONCAT - FACS (BONCAT — Fluorescence - Activated Cell Sorting).

An antibody that works well for Western blots on denaturing gels, for example, should not be presumed to work equally well in nondenaturing assays such as fluorescence - activated cell sorting.

Human neural stem cells are derived via fluorescence - activated cell sorting (FACS) from donated fetal brain tissue.

A fluorescence - activated cell sorting (FACS) has been used for high - throughput cell sorting.

This simple protocol is based on automated fluorescent activated cell sorting (FACS) of OPCs.

Based on this technological advance, we are able to implement the principle of surface free energy - activated cell sorting.»

Next, they used a technique called fluorescence - activated cell sorting to separate out the newly differentiated retinal ganglion cells from a mixture of different cells into a highly purified cell population for study.

Called SIF - seq, for site - specific integration fluorescence - activated cell sorting followed by sequencing, this new technique complements existing genomic tools, such as ChIP - seq (chromatin immunoprecipitation followed by sequencing), and offers some additional benefits.

Fluorescent activated cell sorts were performed using the FACSAria for sort 1 through 15 and the FACSStar PLUS (both BD Biosciences) for sort 16 through 22.

Not exact matches

Fluorescence - activated sorting is then used to identify and retrieve from this mix only those cells that display strong reporter gene expression, which represent the cells with the most active enhancers.

The research provides a sort of rulebook that can be used with high success to track down antigens likely to activate a given T cell, easing a bottleneck that has constrained such studies.

Abbreviations: Aβ, amyloid β - peptide; AD, Alzheimer's disease; ALS, amyotrophic lateral sclerosis; Ambra1, activating molecule in Beclin -1-regulated autophagy; AMPK, AMP - activated protein kinase; APP, amyloid precursor protein; AR, androgen receptor; Atg, autophagy - related; AV, autophagic vacuole; Bcl, B - cell lymphoma; BH3, Bcl - 2 homology 3; CaMKKβ, Ca2 + - dependent protein kinase kinase β; CHMP2B, charged multivesicular body protein 2B; CMA, chaperone - mediated autophagy; 2 ′ 5 ′ ddA, 2 ′, 5 ′ - dideoxyadenosine; deptor, DEP - domain containing mTOR - interacting protein; DRPLA, dentatorubral pallidoluysian atrophy; 4E - BP1, translation initiation factor 4E - binding protein - 1; Epac, exchange protein directly activated by cAMP; ER, endoplasmic reticulum; ERK1 / 2, extracellular - signal - regulated kinase 1/2; ESCRT, endosomal sorting complex required for transport; FAD, familial AD; FDA, U.S. Food and Drug Administration; FIP200, focal adhesion kinase family - interacting protein of 200 kDa; FoxO3, forkhead box O3; FTD, frontotemporal dementia; FTD3, FTD linked to chromosome 3; GAP, GTPase - activating protein; GR, guanidine retinoid; GSK3, glycogen synthase kinase 3; HD, Huntington's disease; hiPSC, human induced pluripotent stem cell; hVps, mammalian vacuolar protein sorting homologue; IKK, inhibitor of nuclear factor κB kinase; IMPase, inositol monophosphatase; IP3R, Ins (1,4,5) P3 receptor; I1R, imidazoline - 1 receptor; JNK1, c - Jun N - terminal kinase 1; LC3, light chain 3; LD, Lafora disease; L - NAME, NG - nitro - L - arginine methyl ester; LRRK2, leucine - rich repeat kinase 2; MIPS, myo - inositol -1-phosphate synthase; mLST8, mammalian lethal with SEC13 protein 8; MND, motor neuron disease; mTOR, mammalian target of rapamycin; mTORC, mTOR complex; MVB, multivesicular body; NAC, N - acetylcysteine; NBR1, neighbour of BRCA1 gene 1; NOS, nitric oxide synthase; p70S6K, ribosomal protein S6 kinase - 1; PD, Parkinson's disease; PDK1, phosphoinositide - dependent kinase 1; PE, phosphatidylethanolamine; PI3K, phosphoinositide 3 - kinase; PI3KC1a, class Ia PI3K; PI3KC3, class III PI3K; PI3KK, PI3K - related protein kinase; PINK1, PTEN - induced kinase 1; PKA, protein kinase A; PLC, phospholipase C; polyQ, polyglutamine; PS, presenilin; PTEN, phosphatase and tensin homologue deleted from chromosome 10; Rag, Ras - related GTP - binding protein; raptor, regulatory - associated protein of mTOR; Rheb, Ras homologue enriched in brain; rictor, rapamycin - insensitive companion of mTOR; SBMA, spinobulbar muscular atrophy; SCA, spinocerebellar ataxia; SLC, solute carrier; SMER, small - molecule enhancer of rapamycin; SMIR, small - molecule inhibitor of rapamycin; SNARE, N - ethylmaleimide - sensitive factor - attachment protein receptor; SOD1, copper / zinc superoxide dismutase 1; TFEB, transcription factor EB; TOR, target of rapamycin; TSC, tuberous sclerosis complex; ULK1, UNC -51-like kinase 1; UVRAG, UV irradiation resistance - associated gene; VAMP, vesicle - associated membrane protein; v - ATPase, vacuolar H + - ATPase; Vps, vacuolar protein sorting