Sentences with phrase «adherent cultures»

(c) Proliferation curves of the parental cells, hypoxia - exposed cells (A3), and the cycling hypoxia - selected subpopulations grown as adherent cultures (top two plots) or as spherical cultures (the third plot).

Since human embryonic stem cells grow in an adherent culture system, for cells being reprogrammed this «new culture system» is an adherent culture system (to try and mimic the conditions the embryonic stem cells want to be happy).

Cells were detached (adherent culture) or dissociated (spherical culture) by trypsin / EDTA and resuspended in PBS for cell counting using the ViaCount assay.

In short, there is no blastocyst - like stage — when the somatic cells are reprogrammed, they're immediately cultured as cells in normal adherent culture.

After 6 — 7 days of suspension culture, the neurospheres were replated onto matrigel - coated 60 mm tissue culture dishes for adherent culture in the same medium for another 2 — 3 days.

Primary neurospheres were then collected and replated onto matrigel - coated tissue culture dishes for adherent culture in the same medium for another 2 — 3 days.

Numerous neural rosettes were formed 2 — 3 days after adherent culture.

Not exact matches

But, to my surprise, I learned through my reporting that as Christianity finds its place in Chinese culture, its adherents are less defined by how they cope with state - sponsored adversity.

Dean Kelley has argued that the conservatives provide a clearer meaning system for their adherents, The culture at large is shifting on several fronts toward a style more prescriptive, less permissive.

(8) Yet rarely has the church addressed this aspect of culture in a sustained and balanced way that helps its adherents live faithful lives within this particular culture.

It provided an ideological framework within which the many religious communities of India as well as the plurality of linguistic caste and ethnic cultures (in the formation of which one or other religions had played a dominant role) could participate together with the adherents of secular ideologies like Liberalism and Socialism (which emerged in India in the framework of the impact of modern humanism of the West mediated through western power and English education).

The mass - culture media religion is so superficial that it scarcely matters whether its adherents are cynically being «taken.»

I'm not personally interested in creation stories that have only small numbers or adherents and little or no impact on the cultures that brought forth the western technological society in which I live.

But that, as Novak points out, relies upon «the erroneous notion that democracy must create its own culture rather than drawing upon the practical wisdom of more primary cultures, cultures like Judaism that inevitably trace their wisdom back to the God who has created all and who has revealed himself to its adherents....

The question modern readers have to answer is whether the Greco - Roman household codes reflected upon in Ephesians, Colossians, and 1 Peter are in and of themselves holy and divinely instituted, or if their appearance in Scripture represents the early church's attempt to blend Christianity and culture in such a way that it would preserve the dignity of adherents while honoring prevailing social and legal norms of the day.

The fact that, in spite of its early association with that world and of the antagonism felt because of this seeming alliance, Christianity won some adherents from other cultures is evidence of the striking universality and vigor of the impulse which created it.

When theology is insulated from broader conversations, it can lead to a culture of complacency wherein adherents can slavishly regurgitate doctrinal «facts» with gusto (but without reflection), yet at the same time experience a theological and spiritual impoverishment.

The Minister of Information and Culture, Alhaji Lai Mohammed, has enjoined the adherents of the two major religions in Nigeria to imbibe the spirit of harmony and tolerance in order to strengthen the bond of unity across the country.

The Minister of Information and Culture, Alhaji Lai Mohammed, has enjoined the adherents of the two major religions in Nigeria to imbibe the spirit...

The scientists then tried the proline / polyproline combination in a test system of adherent cell cultures that were subjected to freeze - thaw conditions.

GenIbet offers 1000 m2 of GMP - compliant upstream and downstream manufacturing facilities, including QC labs and R&D facilities, three BSL2 units for the cultivation of bacterial cells (50 L capacity), insect and mammalian cells (50 L capacity for suspension cell cultures, 168,000 cm2 capacity for adherent cells) and the propagation of viruses (50 L capacity, with space to expand up to 200 L).

Bone marrow - derived mesenchymal stem cells (BM - MSCs) are obtained by isolating mononucleated cells from a marrow aspirate by centrifugation on a density gradient, followed by adherent cell expansion in tissue culture.

Furthermore, we found that the stem - like and EMT phenotypes observed in the cycling hypoxia - selected subpopulation are not reversible, because we obtained the same molecular profile from this subpopulation by culturing them as spherical cultures for several months in normal oxygen content and culturing them as adherent cells (Figure S2 in Additional file 2).

Viral Manufacturing To manufacture viral vectors, two approaches generally are adopted for culturing purposes: scale - out of adherent - cell systems based on 2D planar technologies and scale - up of 3D suspension cell cultures in stirred - tank bioreactors.

Hypoxic effect on adherent holoclone culture (C) and percentage ALDH1 - positive cells (D).

Non-adherent cells are removed after overnight culture and adherent populations are expanded under low density conditions (seeding density 1000 cells / cm2 and allowed to grow upto 60 — 70 % confluency).

Since the OASIS was primarily conceived by James Halliday (Mark Rylance), an eccentric inventor with a vast nostalgia for the pop culture of the 1980s, Halliday's most ardent adherents, most of whom weren't even born then, are awash in the movie and video game artifacts of that era.

The logic of the argument that it is the Asian culture or something «beyond their textbooks» that produces math success leads to the conclusion that, as NCTM adherents often contend, content doesn't matter nearly as much as the teacher or the culture that produces the «proper conditions for learning.»

Our guides are specifically selected and trained to be expertly qualified, intimate with the wildlife, geography, history and culture of our locale, strictly adherent to minimum impact wildlife viewing and respectful of the environment.

== > The second analysis, mine, demonstrates that the concept of a «climate culture» provides a much better fit to the data, a culture that has adherents in its own right plus asymmetrical alliance with politics; also that Kahan's conclusion is largely a product of his own bias due to a major influence from this same climate culture.

The a) group are ideologically committed to the concept of climate calamity, and hence happen to be Dem / Libs as well as adherents to climate culture.

Typically most adherents of a culture are honestly motivated, so the clues in section 4 aren't useful.

Cultures often create the image of a powerful enemy from the thinnest of contexts; not only motivates the adherents to angry passion for all that the culture stands for (which is all that the enemy is not), but the active force of the enemy apparently unraveling and undermining all the good works, helps explain why there is little or no progress to Nirvana.

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