Sentences with phrase «adult fibroblast»

The generation of induced pluripotent stem cells from adult fibroblast cells to create iPS cells.

And so again, kind of using what the embryo normally does to develop but transferring that into an adult fibroblast and making that tissue - and it's very, very fascinating research.

«Induction of Pluripotent Stem Cells From Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors,» by Kazutoshi Takahashi and Shinya Yamanaka, Cell, Aug. 25, 2006.

The novel reprogramming approach employed the retroviral transduction of Oct4, Sox2, and Klf4 in mouse embryonic and adult fibroblasts followed by treatment with the GSK3β Inhibitor BIO and the pleiotropic cytokine Oncostatin M (OSM).

Embryonic cardiac fibroblasts promote myocyte proliferation, while adult fibroblasts promote myocyte hypertrophy,» said Masaki Ieda, MD, PhD, Gladstone postdoctoral fellow and lead author on the study.

It has also been reported that Kap1 controls endogenous retroelements during early embryonic development and in embryonic stem cells but not in adult fibroblasts [45].

Not exact matches

Repeated transfection of two expression plasmids, one containing the complementary DNAs (cDNAs) of Oct3 / 4, Sox2, and Klf4 and the other containing the c - Myc cDNA, into mouse embryonic fibroblasts resulted in iPS cells without evidence of plasmid integration, which produced teratomas when transplanted into mice and contributed to adult chimeras.

The research team took skin fibroblast tissue from adult mole - rats and reprogrammed the cells to revert to pluripotent stem cells.

Skin - producing cells called fibroblasts from the tip of an adult mouse's tail have been reprogrammed to make eggs, Japanese researchers report online October 17 in Nature.

Imagine researchers» excitement, then, when Kyoto University's Shinya Yamanaka reported last year that his group had created embryolike cells directly, without transferring chromosomes, simply by injecting mouse fibroblast cells with four genes that are active in embryonic cells but not in adult ones.

A structure containing interstitial fibroblast - like cells developed within the WAT in sexually immature migratory adults.

First, the MimEX ™ Cell Plating Procedure is used to maintain a uniform irradiated fibroblast monolayer for the rapid clonal expansion and efficient differentiation of tissue - specific adult epithelial stem cells.

Troubleshooting fibroblast contamination when isolating adult stem cells from biopsy tissue

Induction of stem cell gene expression in adult human fibroblasts without transgenes.

Having shown that it's possible to directly reprogram mouse fibroblasts (in a dish and in adult hearts) and also human heart fibroblasts in a dish, Dr. Srivastava's group is testing the recipe in pigs, whose heart size and physiology is closer to our own.

Pfisterer U, Wood J, Nihlberg K, Hallgren O, Bjermer L, Westergren - Thorsson G, Lindvall O, Parmar M.Efficient induction of functional neurons from adult human fibroblasts

Yamanaka's group used human adult dermal fibroblasts and induced them to become iPSCs, appearing and functioning like hESCs, by having them express the same proteins as he used with mouse cells: Oct - 4, Sox2, Klf4, and c - Myc (Takahashi et al., 2007).

They made adult fibroblastic mouse cells become essentially mouse embryonic stem cells, in appearance and function, by forcing the fibroblasts to express four key embryonic stem cell factors: Oct - 4, Sox2, Klf4, and c - Myc.

Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., and Yamanaka, S. Induction of Pluripotent Stem Cells from Adult Human Fibroblasts.

By observing the cells in this system, they found that the embryonic cardiac fibroblasts promoted cell division by the cardiomyocytes more efficiently than adult cardiac fibroblasts.

«We found a major difference in the function of embryonic and adult cardiac fibroblasts.

By this way, they unravelled the mechanisms of cellular fate during developmental growth of dermal skin, and especially investigated how fibroblast of the upper and lower dermal lineages contribute to the wound healing in adult skin.

Pluripotent stem cells can be generated from adult mouse - tail tip fibroblasts and adult human fibroblasts by the retrovirus - mediated transfection of four transcription factors, Oct3 / 4, Sox2, c - Myc, and Klf4.

Fibroblast growth factor - 2 activates a latent neurogenic program in neural stem cells from diverse regions of the adult CNS.

The adult human heart is only half muscle; the other half is made of support cells called fibroblasts that help repair wounds and form scars around damaged tissue.

We derived a fibroblast line from a skin biopsy from a healthy adult male (HUF1)(Figure 1A) and used immunocytochemistry to characterize the expression of cell surface markers commonly expressed on pluripotent stem cells (Figure 1B, C and D).

(A — B) Primary adult human fibroblast line HUF1 (A) Phase contrast image (B) Immunocytochemical detection of SSEA3 expression (green).

Eight additional primary adult human fibroblast lines were derived from skin biopsies and immunoassayed.

Methods to enhance the reprogramming efficiency would significantly increase the feasibility of this approach, especially for cell types which tend to be more difficult to reprogram, such as the primary adult human fibroblasts used in this study.