Sentences with phrase «adult fibroblast cells»
The generation of induced pluripotent stem cells from adult fibroblast cells to create iPS cells.
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Repeated transfection of two expression plasmids, one containing the complementary DNAs (cDNAs) of Oct3 / 4, Sox2, and Klf4 and the other containing the c - Myc cDNA, into mouse embryonic fibroblasts resulted in iPS cells without evidence of plasmid integration, which produced teratomas when transplanted into mice and contributed to adult chimeras.
The research team took skin fibroblast tissue from adult mole - rats and reprogrammed the cells to revert to pluripotent stem cells.
Skin - producing cells called fibroblasts from the tip of an adult mouse's tail have been reprogrammed to make eggs, Japanese researchers report online October 17 in Nature.
Imagine researchers» excitement, then, when Kyoto University's Shinya Yamanaka reported last year that his group had created embryolike cells directly, without transferring chromosomes, simply by injecting mouse fibroblast cells with four genes that are active in embryonic cells but not in adult ones.
A structure containing interstitial fibroblast - like cells developed within the WAT in sexually immature migratory adults.
First, the MimEX ™ Cell Plating Procedure is used to maintain a uniform irradiated fibroblast monolayer for the rapid clonal expansion and efficient differentiation of tissue - specific adult epithelial stem cells.
Troubleshooting fibroblast contamination when isolating adult stem cells from biopsy tissue
«Induction of Pluripotent Stem Cells From Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors,» by Kazutoshi Takahashi and Shinya Yamanaka, Cell, Aug. 25, 2006.
Induction of stem cell gene expression in adult human fibroblasts without transgenes.
Yamanaka's group used human adult dermal fibroblasts and induced them to become iPSCs, appearing and functioning like hESCs, by having them express the same proteins as he used with mouse cells: Oct - 4, Sox2, Klf4, and c - Myc (Takahashi et al., 2007).
They made adult fibroblastic mouse cells become essentially mouse embryonic stem cells, in appearance and function, by forcing the fibroblasts to express four key embryonic stem cell factors: Oct - 4, Sox2, Klf4, and c - Myc.
Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., and Yamanaka, S. Induction of Pluripotent Stem Cells from Adult Human Fibroblasts.
By observing the cells in this system, they found that the embryonic cardiac fibroblasts promoted cell division by the cardiomyocytes more efficiently than adult cardiac fibroblasts.
Pluripotent stem cells can be generated from adult mouse - tail tip fibroblasts and adult human fibroblasts by the retrovirus - mediated transfection of four transcription factors, Oct3 / 4, Sox2, c - Myc, and Klf4.
Fibroblast growth factor - 2 activates a latent neurogenic program in neural stem cells from diverse regions of the adult CNS.
The adult human heart is only half muscle; the other half is made of support cells called fibroblasts that help repair wounds and form scars around damaged tissue.
We derived a fibroblast line from a skin biopsy from a healthy adult male (HUF1)(Figure 1A) and used immunocytochemistry to characterize the expression of cell surface markers commonly expressed on pluripotent stem cells (Figure 1B, C and D).
It has also been reported that Kap1 controls endogenous retroelements during early embryonic development and in embryonic stem cells but not in adult fibroblasts [45].
Methods to enhance the reprogramming efficiency would significantly increase the feasibility of this approach, especially for cell types which tend to be more difficult to reprogram, such as the primary adult human fibroblasts used in this study.