We collected sample sets of
adult worker honey
bees from several areas and years: (1 — 2) Two initial sample sets of
adult honey
bees from CCD colonies were obtained in 2006 — 2007 from twelve beekeeping operations from western, northeastern, and southeastern regions of the USA, (3) Samples from packages of imported Australian
bees provided a non-CCD 2007 reference, (4)
bees sampled in 2008 from a large, non-migratory beekeeping operation in northwestern Montana with no history of CCD provided a second reference set, (5)
bee samples obtained in 2009 from a Florida apiary when 500 colonies suddenly collapsed constituted an independent CCD sample set by location and year.