"Affinity purification" is a process used in science to separate and purify specific molecules or proteins. It involves using a substance or material that has a strong attraction or affinity towards the target molecules, which helps to pull them out from a mixture and isolate them for further analysis or use.
Full definition
Tissue - specific mRNAs are isolated following ribosome
affinity purification of tissue - specific tagged ribosomal proteins followed by polysomal profiling.
Instead, we are using microglia - specific translating
ribosome affinity purification (TRAP) technology developed here at Mt. Sinai Icahn School of Medicine by Dr. Anne Schaefer.
The InterPlay Adenoviral TAP System combines our unique
tandem affinity purification (TAP) system with our exclusive adenoviral gene delivery system, the AdEasy Adenoviral Vector System, for enhanced gene delivery to a broader range of mam... Read more...
Conventional isolation methods — ultracentrifugation,
affinity purification using antibodies to surface antigens, and precipitation with polymer reagents — are not fully satisfactory for recovery efficiency, purity, and operability.
Immunoprecipitation (IP), mass spectrometry (MS), co-immunoprecipitation (Co-IP) and / or
affinity purification investigate more aspects including posttranslational modifications (PTMs), DNA... — Read more
The facility provides production of recombinant proteins in insect cell systems and
affinity purification of recombinant proteins upon request.
Julia is also recognized for her role as a technology innovator in plant biology; her development of the «translating
ribosome affinity purification «TRAP» method has opened a window into the «translatome».
May be used for
the affinity purification of the SIV - Pk fusion protein from bacterial expression vectors 282 and 283.