Conventional isolation methods — ultracentrifugation,
affinity purification using antibodies to surface antigens, and precipitation with polymer reagents — are not fully satisfactory for recovery efficiency, purity, and operability.
Not exact matches
May be
used for the
affinity purification of the SIV - Pk fusion protein from bacterial expression vectors 282 and 283.
Adam
used a wide range of techniques revolving around construction of His - Tag plasmids and eventual protein
purification via Ni - NTA
affinity and reverse phase HPLC.
Protein complexes isolated through
affinity purification of a bait protein
using an epitope tag and competitive elution are separated through blue native electrophoresis.