Sentences with phrase «after electrophoresis»

PCR products were visualized by blue light after electrophoresis on a 2.5 % agarose gel containing 1 × GelStar ® Nucleic Acid Gel Stain (Lonza, Breda, The Netherlands) in 1 × Tris - borate buffer (pH 8.0).

The gelatinolytic assays were carried out in two different ways to obtain complementary information: firstly, cells were treated or not with the test compound and samples from these were submitted to gelatinase zymography to detect the effects of the kahweol treatment on the expression of gelatinase activities; secondly, in some experiments, samples from control, untreated HT - 1080 fibrosarcoma cells were submitted to zymography and, after electrophoresis, different concentrations of kahweol were added to the substrate buffer to determine the potential direct effect of kahweol on gelatinase activity.

Not exact matches

The appearance of the variant after starch - gel electrophoresis of the hemolysates supports the concept that the lactic dehydrogenase isozymes are determined by the interaction of two subunits which are under separate genetic control.

After waiting for the chemical reactions to take place, we take my CRISPR plasmid to an electrophoresis machine, a tray that has wires hooked to it.

After a few days, we isolate the DNA from the cells, amplify it with the polymerase chain reaction, and use electrophoresis to show that the CD32 gene has been cut into pieces.

After a traditional PCR has been completed, the data are analyzed by resolution through an agarose gel or, more recently, through a capillary electrophoresis system.