Sentences with phrase «after hybridization»

Edison tested about seventeen thousand different plants before settling on a type of Goldenrod weed which produced 5 % latex in its native form and 12 % after hybridization.

After hybridization, arrays were scanned with a GenePix 4000B scanner (Axon Instruments).

Those variants entered the human population after hybridization.

Not exact matches

The researchers then compared survival rates and physical characteristics to determine whether hybridization affects a fish's potential to adapt after multiple generations of natural selection in the wild.

After acetylation by treatment with 0.1 m triethanolamine 0.25 % acetic anhydride, pH 8.0, the tissue was prehybridized (prehybridization solution: 0.75 m NaCl, 25 mm PIPES, 25 mm EDTA, 5 × Denhardt's reagent, 0.2 % SDS, 250 μg / ml Escherichia coli tRNA, and 500 μg / ml salmon testis DNA, pH 6.8) at room temperature for 5 h. Riboprobes were incubated for 10 min at 85 °C before application onto the sections and used for hybridization at a final concentration of 500 ng / ml.

We sampled the bees from the surface of the bee ball at 0, 30, and 60 min after formation of the bee ball and examined the Acks - expressing brain regions using in situ hybridization (n = 5, 7 and 7 for 0, 30 and 60 min, respectively).

(D — O) In situ hybridization of Acks in each brain area shown in (B) in the brains of workers 0 (D, G, J, and M), 30 (E, H, K, and N) and 60 min (F, I, L, and O) after formation of the bee ball.

(D — R) In situ hybridization of Acks in each brain area shown in (B) in the brains of workers at 0 (D, G, J, M, and P), 30 (E, H, K, N, and Q) and 60 min (F, I, L, O, and R) after the bee ball formation.

When in situ hybridization was performed with sections corresponding to the middle (Figure 4, Figure S1, and Figure S2), more rostral (Figure S3) and more caudal brain parts (Figure S4), the Acks signals were most densely detected in the Class II KCs at 30 and 60 min after the bee ball formation (Figure 4H, I, K, and L, Figure S3H, I, K, and L, and Figure S4H, I, K, and L), whereas they were scarcely detected at 0 min (Figure 4G and J, Figure S3G and J, and Figure S4G and J).

Statistical analyses of the number of Acks signals detected in in situ hybridization experiments using sections corresponding to the middle brain parts (n = 5, 5, and 4 for 0, 30, and 60 min, respectively) revealed that there was a significant increase in the number of Acks signals at 30 and 60 min after the bee ball formation in both the Class I and the Class II KCs, and at 60 min after the bee ball formation in the brain area between the DLs and the OLs, and in the OLs (Figure 4S).

(D — R) In situ hybridization of Acks in each brain area shown in (B) in the brains of workers at 0 (D, G, J, M, and P), 30 (E, H, K, N, and Q), and 60 min (F, I, L, O, and R) after bee ball formation.

One of the most striking aspects of this study is that hybridization between two distinct species led to the development of a new lineage that after only two generations behaved as any other species of Darwin's finches, explained Leif Andersson, a professor at Uppsala University who is also affiliated with the Swedish University of Agricultural Sciences and Texas A&M University.

The unique location, large size, and specific arrangement of the Platynereis mesodermal midline cells allowed their unambiguous identification after whole - mount in situ hybridization (WMISH) and thus expression profiling by confocal imaging.

The current secondary contact is thought to result from a range expansion associated with the amelioration of climatic conditions after the last glacial maximum (∼ 18000 ya)[20], although previous glacial / interglacial cycles during the Pleistocene may have provided multiple opportunities for vicariant and past hybridization events.

We wrote about this hybrid vehicle over a year ago as it was pending European launch as a 2012 model, after much hemming, hawing, and well - documented reluctance from years prior to hybridization by the division of the Volkswagen Group.