The term "irradiation" refers to the process of exposing an object or substance to ionizing radiation, such as X-rays or gamma rays.
Full definition
Phillips and colleagues [47] reported that the non-adherent population of monolayer cultures of breast cancer cells has the ability to initiate mammosphere
formation after irradiation.
Compared to MEF cells, which can be maintained for only 5 — 7
days after irradiation, HFF cells as a feeder layer avoid frequent passage and allow the maintenance of established cell - cell interaction during the reprogramming process.
Residual or recurrent laryngeal
cancer after irradiation is a difficult clinical problem with a rate that ranges from 13 % to 36 % of cases.
Thus, it is desirable to have feeder cells which can survive for an extended period of time to support the derivation of iPS
cells after irradiation.
After irradiation, cells were further incubated with drugs for either 4 or 24 h depending on the experimental requirement.
After irradiation, they were able to maintain the typical morphology for up to two weeks, a capacity which is especially advantageous for iPS cell derivation.