Sentences with phrase «after overnight culture»

Non-adherent cells are removed after overnight culture and adherent populations are expanded under low density conditions (seeding density 1000 cells / cm2 and allowed to grow upto 60 — 70 % confluency).

After overnight culture, they were treated with a range of concentrations of ActD for 24 and 48 h, followed by incubation with methylthiazolyldiphenyl - tetrazolium bromide (MTT)(Sigma) for the assay.

Not exact matches

A day after deadly violence struck the J'ouvert parade in Brooklyn for the third straight year, Mayor Bill de Blasio said on Tuesday that the annual celebration of Caribbean culture would continue but that the city would consider changing the hours of the overnight event, which precedes the much bigger West Indian American Day Parade.