Sentences with phrase «after photobleaching»
Both are capable of collecting transmitted light as well as epifluorescence data and can be used for time - lapse imaging, Fluorescence Resonance Energy Transfer (FRET), and Fluorescence Recovery After Photobleaching (FRAP) experiments.
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Fluorescence recovery after photobleaching of GFP - tagged wild - type and S39A fascin showed that dephosphorylated fascin underwent rapid cycles of association to and dissociation from actin filaments in filopodia, with t (1/2) < 10 s.
JQ1 inhibits recruitment and binding of Brd4 to TNFα and E-selectin promoter elements, and accelerates recovery time in FRAP (fluorescence recovery after photobleaching) assays using GFP - Brd4.
In FRAP assays (FRAP: Fluorescence Recovery After Photobleaching), the time that fluorescent molecules need to replenish a bleached - out area is measured,...
The Fluorescence Recovery After Photobleaching (FRAP) assay has been used to validate many of the SGC's chemical probes against bromodomains.
These include methods for deconvolution in fluorescence microscopy as applied to cellular morphogenesis, in addition to methods for live - cell binding measurements using fluorescence recovery after photobleaching (FRAP), fluorescence correlation spectroscopy (FCS) and single molecule tracking (SMT) as applied to transcription factor regulation.
Scientists are therefore using so - called «FRAP» assays (FRAP: Fluorescence Recovery After Photobleaching) to investigate diffusion kinetics, a method established more than 40 years ago.