Sentences with phrase «after transfection»

Retroviral or lentiviral supernatants were collected and filtered 24 and 48 hours after transfection.

B, Small iPSC colonies can be detected 10 days after transfection with pCEP - Oct4 and pCEP - Nanog.

«To reduce off - target effects, we introduced preassembled Cpf1 RNPs into cells, assuming that Cpf1 RNPs, similar to Cas9 RNPs, would cleave target sites immediately after transfection and would be degraded rapidly by endogenous enzymes that break the proteins, thereby reducing off - target effects without sacrificing on - target effects.»

48 hr after transfection, cells were seeded into six - well plates in triplicates and selected with puromycin (1.5 μg / ml) for 3 days.

Transfected cells were trypsinized and re-plated into 96 - well plates 20 — 24 hours after transfection.

Lysates were harvested 3 days after transfection and subjected to immunoblotting.

Virus - containing supernatants were collected forty - eight hours after transfection, passed through a 0.45 µm filter (Millipore) and concentrated by ultracentrifugation at 100,000 g for two hours at 4 °C.

Supernatants were collected for 3 consecutive days beginning 48 hours after transfection.

Forty - eight hours after transfection, the media were centrifuged and the supernatant was diluted with culture medium to 100 %, 50 % and 25 % the original concentrations.

Forty - eight hours after transfection, the media were centrifuged at 800 g for 3 min and the supernatant was transferred into new tubes.

Twelve hours after transfection, cells were treated with or without IFNα for another 24 h.

Twelve hours after transfection, the cells were incubated for another 24 h in the culture supernatants of NB4 cells treated with or without ATRA.

Twenty - four hours after transfection, cells were treated with the different drugs for 24 — 36 hours.

Twelve hours after transfection, cells were treated with or without 1,000 units / mL IFNα.

The luciferase activity was measured 36 h after transfection.

Six hours after transfection 2.5 uM SAHA (to increase global histone acetylation) was added and BAZ2 - ICR was added 1 hour before imaging, which was carried out 24 hours after transfection.

Thirty - six hours after transfection, the culture supernatants were collected and used for IFNα titration.

72 h after transfection, cells were collected for RFLP assay (Mutation frequency analysis) or were seeded individually into 96 - well plates for isolating single cell colonies.

Twelve hours after transfection, cells were treated with or without IFNα for another 24 h. Cell lysates (Input) were then immunoprecipitated (IP) with anti — IRF - 9 antibody followed by Western blot (WB) analysis with anti-STAT2 antibody.

NSCs transfected with anti-HER2 antibody were used 24 h after transfection, the time - point that correlated with peak antibody expression.