Sentences with phrase «after transfection with»
(B) Neurite length of GDF5 - treated (200 ng / ml daily for 72 h) SH - SY5Y cells at 72 h. (C — F) p - Smad1 / 5 levels as determined by (C, D) Western blots or (E, F) immunocytochemistry in SH - SY5Y cells 24 h after transfection with control miRNA or miR - 181a inhibitor.
http://www.neuronalsignaling.org/
B, Small iPSC colonies can be detected 10 days after transfection with pCEP - Oct4 and pCEP - Nanog.
Not exact matches
After 48 hours of transfection, cell extracts were prepared and the expression level of FLAG - DDX3, p21waf1 / cip1, and α - actin was detected by immunoblotting with anti-FLAG, anti-p21waf1 / cip1, and anti-α-actin (Santa Cruz Biotechnology, Santa Cruz, CA) antibodies.
Forty - eight hours after transfection, the media were centrifuged and the supernatant was diluted with culture medium to 100 %, 50 % and 25 % the original concentrations.
Twenty - four hours after transfection, cells were treated with the different drugs for 24 — 36 hours.
Twelve hours after transfection, cells were treated with or without 1,000 units / mL IFNα.
Two days after infection / transfection, cells were plated on mitotically inactivated MEFs (Chemicon), with hESCs medium, in the presence or not of 50 µg / ml of hygromycin B (Invitrogen).
Twelve hours after transfection, cells were treated with or without IFNα for another 24 h. Cell lysates (Input) were then immunoprecipitated (IP) with anti — IRF - 9 antibody followed by Western blot (WB) analysis with anti-STAT2 antibody.
48 hours after transfection, cells were lysed with 100 µL / well 1 × passive lysis buffer (PLB, Promega) for 15 minutes with shaking.
Anti-HIF-1-alpha unpurified antibody (ab51608) reactivity with reduced Hep3B cell lysate after transient transfection of scrambled siRNA (lanes1 - 3 and 7 - 9) or HIF -1-alpha siRNA (lanes 4 - 6 and 10 - 12).
NSCs transfected with anti-HER2 antibody were used 24 h after transfection, the time - point that correlated with peak antibody expression.
48 hr after transfection, cells were seeded into six - well plates in triplicates and selected with puromycin (1.5 μg / ml) for 3 days.