Sentences with phrase «agarose cyrogel»

The SLC6A4 promoter VNTR polymorphism was genotyped by agarose gel size fractionation as described in ref.

Responsible and highly motivated professional with solid and diverse experience as Molecular Biologist with excellence in isolating and purifying DNA, bacterial plating, polyacrylamide gel electrophoresis, agarose gel electrophoresis.

Performed various tasks in the lab such as preparing agarose gels and buffer dilutions for electrophoresis.

Analytical tools: Thin layer chromatography, UV / Visible spectrophotometry, microcentrifuge, metabolism chamber, compound microscope, dissecting microscope, SDS - PAGE, PCR, agarose gel electrophoresis.

Products were resolved on a 2 % agarose gel.

The supernatant was mixed with 300 µl of Ni - NTA agarose (Qiagen), and the proteins were allowed to bind for 3 hours, followed by washing with 20 ml of resuspension buffer, and eluted with 500 µl of elution buffer (20 mM NaHPO4, 300 mM NaCl, 10 % glycerol, 150 mM Imidazole, pH 7.8).

PCR products were visualized by blue light after electrophoresis on a 2.5 % agarose gel containing 1 × GelStar ® Nucleic Acid Gel Stain (Lonza, Breda, The Netherlands) in 1 × Tris - borate buffer (pH 8.0).

This product was similarly purified in a low - melt agarose gel and used at 1 ng / μl along with 50 ng / μl of the unc - 36 (+) cosmid derivative RIp16 (gift of L Lobel) for microinjection into unc - 36 -LRB--) animals.

At 24 hours post fertilization (hpf), fluorescent embryos were dechorionated, mounted on coverslips in 1.2 % ultra-low gelling agarose, overlaid with 30 % Danieua / PTU and analyzed by laser scanning confocal microscopy (Zeiss LSM510 Meta).

To generate a full - length unc - 9:: gfp construct, the 14.7 - kb UNC - 9A + B PCR product (representing the 5» end of unc - 9) and the unc - 9:: gfp plasmid were digested separately with Kpn I, digests electrophoresed through low - melt agarose (SeaPlaque GTG agarose, FMC, Rockland, ME, USA), and appropriate fragments purified by phenol extraction and ethanol precipitation.

The resulting PCR amplification products were analyzed by electrophoresis in 1.5 % agarose gels.

PCR products were separated by gel electrophoresis on 2 % agarose gel.

DNA libraries were then re-amplified for another 13 cycles in quintuplicates or sextuplicates, followed by pooling and purification, visual inspection on a 3.5 % agarose gel, and final quantification using a NanoDrop 2000c spectrophotometer (FisherScientific).

c, Southern blot (left) membrane hybridization of 10 µg of BamHI - digested genomic DNA (see corresponding agarose gel on right) using a DNA probe from the pCEP backbone.

Genomic DNA of clones B31 - A3, ospC7, and ospC7 / ospC +4 was separated through agarose gels, transferred to a membrane, and hybridized with a 32P - labeled probe specific for ospC.

This activity introduces DNA analysis through DNA digestion with restriction enzymes and agarose gel electrophoresis.

The resulting PCR products were separated by agarose gel electrophoresis.

Digested DNA was visualized by agarose gel (1.5 — 2.0 %).

After a traditional PCR has been completed, the data are analyzed by resolution through an agarose gel or, more recently, through a capillary electrophoresis system.

The amplified DNA products were analyzed on a 1.0 % agarose gel, and pieces of gel containing the amplified DNA bands were excised and purified using a gel extraction kit (Takara Bio).

Using acrylamide instead of agarose gives much sharper bands.

PCR products were visualized by electrophoresis in an ethidium bromide - stained 1.8 % agarose gel.

Amplicons were visualized in a 2 % agarose gel stained with Ethidium Bromide and purification was performed directly from the amplification reaction using the Qiagen PCR purification Kit according to the manufacturer's instructions.

PCR products were separated by electrophoresis on a 2 % agarose gel, stained with ethidium bromide and visualized by UV illumination.

If you are analyzing short DNA fragments, run PAGE instead of agarose to get sharper bands.

Immune complexes incubated with protein A agarose slurry containing tRNA for 1 hr at 4 °C with rotation.

Immune complexes were than isolated with E.coli tRNA / Protein A agarose beads, and the obtained purified DNA with subjected to qPCR using primers for HMOX1 E2 promoter.

Ten - microliter aliquots of the nested PCR products were subjected to RFLP analysis by digestion with 2 U of either HinfI or MseI, and digested fragments were resolved by agarose gel electrophoresis in TBE buffer, as described elsewhere [19, 28].

Cell extracts (1.5 mg) were incubated with 15 μL of anti-FLAG M2 - agarose affinity gel (Sigma - Aldrich, St. Louis, MO).

The improved size resolution of the DNA chip allows students to identify their genotype, something impossible with agarose gel electrophoresis.

(This is an updated version of the current DNA Fingerprinting field trip, replacing agarose gels with the DNA chip.)

The resulting DNA fragments are analyzed by agarose gel electrophoresis.

Clade A recombinant nef - protein, expressed as His - tag fusion protein in E. Coli using expression vector pET24 and purified using Ni - agarose columns

Clade C recombinant nef protein, expressed as His - tag fusion protein in E. coli using expression vector pET24 and purified using Ni - agarose columns.

Using chondroitin sulfate to improve the viability and biosynthesis of chondrocytes encapsulated in interpenetrating network (IPN) hydrogels of agarose and poly (ethylene glycol) diacrylate.

Treated cell suspensions were over-layered in agarose coated slides as described [19].

The bioactivity of agarose — PEGDA interpenetrating network hydrogels with covalently immobilized RGD peptides and physically entrapped aggrecan.

Preparation and characterization of superporous agarose — reticulated vitreous carbon electrodes as platforms for electrochemical bioassays.

Assessing neural stem cell motility using an agarose gel - based microfluidic device.

Clade B recombinant Nef protein, expressed as a His - tag fusion protein in E. coli using expression vector pET24 and purified using Ni - agarose columns.

A 3 - ml overlay consisting of EMEM with 0.4 % agarose was added, and the cells were incubated at 37 °C for 72 hours.

When an electric current is applied along the length of the gel, the DNA starts to migrate through the agarose thicket.

Amplified sequences were visualized by gel electrophoresis in 2 % agarose gels stained with GelRed (Biotium).

Conditions for the PCR reaction were 40 cycles of 94 °C for 30 s, 55 °C for 30 s, and 72 °C for 30 s. Amplicons were purified on a 2 % agarose gel, cloned into plasmid vectors using TOPO TA (Invitrogen, Carlsbad, CA), and sent to an outside company (Elim Biopharmaceuticals, Hayward, CA) for Sanger sequencing in both directions using vector primers M13F and M13R.

After cells held in suspension in an agarose solution are grown around the vascular structure, a solvent can be used to wash the sugar away.

First, they 3 - D - printed wormlike strands of a gel called agarose, each serving as a cast of a tiny blood vessel.

Runtimes are approximately one hour versus up to 16 hours needed for methods such as agarose - based, pulsed - field gel electrophoresis.

To imitate the cactus root and its outer covering, they made a material composed of cellulose fibers, agarose cyrogel and microparticles.

The molds therefore were made from agarose so that cells wouldn't stick to the sides or bottom.

He and his colleagues wanted to avoid the classic light - sheet arrangement in which the sample is embedded in a tube of agarose and surrounded by lasers and cameras.