Sentences with phrase «agarose gel»
Agarose gel is a material used in science to separate and study DNA or proteins. It works like a gelatin made from seaweed, which helps to catch and hold these molecules, so scientists can analyze them easily. Full definition
This is 1 million times more sensitive than the typical concentration for an analysis on agarose gel.
sciencedaily.com
Amplified sequences were visualized by gel electrophoresis in 2 % agarose gels stained with GelRed (Biotium).
The «prize,» placed at the exit of each maze, was a lump of agarose gel soaked in hydrochloric acid.
Amplified libraries were size - selected on a 2 % agarose gel at approximately 350 bp average length and then sent to an outside company (Elim Biopharmaceuticals, Hayward, CA) for deep sequencing on an Illumina Genome Analyzer IIx (Illumina, San Diego, CA).
(This is an updated version of the current DNA Fingerprinting field trip, replacing agarose gels with the DNA chip.)
This product was similarly purified in a low - melt agarose gel and used at 1 ng / μl along with 50 ng / μl of the unc - 36 (+) cosmid derivative RIp16 (gift of L Lobel) for microinjection into unc - 36 -LRB--) animals.
PCR products were resolved on a 2 % agarose gel alongside a 100 bp DNA ladder (Promega).
PCR products were visualized by blue light after electrophoresis on a 2.5 % agarose gel containing 1 × GelStar ® Nucleic Acid Gel Stain (Lonza, Breda, The Netherlands) in 1 × Tris - borate buffer (pH 8.0).
Performed various tasks in the lab such as preparing agarose gels and buffer dilutions for electrophoresis.
The SLC6A4 promoter VNTR polymorphism was genotyped by agarose gel size fractionation as described in ref.
The improved size resolution of the DNA chip allows students to identify their genotype, something impossible with agarose gel electrophoresis.
Amplicons were visualized in a 2 % agarose gel stained with Ethidium Bromide and purification was performed directly from the amplification reaction using the Qiagen PCR purification Kit according to the manufacturer's instructions.
DNA integrity is assessed on agarose gel.
The resulting DNA fragments are analyzed by agarose gel electrophoresis.
Following PCR amplification the PCR product was analyzed on a 2 % agarose gel and then extracted and gel purified using Wizard SV Gel and PCR Clean - Up System (Promega).
Conditions for the PCR reaction were 40 cycles of 94 °C for 30 s, 55 °C for 30 s, and 72 °C for 30 s. Amplicons were purified on a 2 % agarose gel, cloned into plasmid vectors using TOPO TA (Invitrogen, Carlsbad, CA), and sent to an outside company (Elim Biopharmaceuticals, Hayward, CA) for Sanger sequencing in both directions using vector primers M13F and M13R.
State - of - the - art DNA chip analyzers can resolve DNA sizes far more accurately than possible with agarose gel electrophoresis.
The amplified DNA products were analyzed on a 1.0 % agarose gel, and pieces of gel containing the amplified DNA bands were excised and purified using a gel extraction kit (Takara Bio).
PCR products were visualized by electrophoresis in an ethidium bromide - stained 1.8 % agarose gel.
PCR products were separated by electrophoresis on a 2 % agarose gel, stained with ethidium bromide and visualized by UV illumination.
After a traditional PCR has been completed, the data are analyzed by resolution through an agarose gel or, more recently, through a capillary electrophoresis system.
Digested DNA was visualized by agarose gel (1.5 — 2.0 %).
The RNA quality has been properly evaluated and provide an agarose gel image with the RNA samples or you may request that the ncRNA Program provide a quality check on the Agilent Bioanalyzer 2100
This activity introduces DNA analysis through DNA digestion with restriction enzymes and agarose gel electrophoresis.
Products were resolved on a 2 % agarose gel.
Analytical tools: Thin layer chromatography, UV / Visible spectrophotometry, microcentrifuge, metabolism chamber, compound microscope, dissecting microscope, SDS - PAGE, PCR, agarose gel electrophoresis.
Core Competencies Organizational Leadership • Research & Analysis • Bacterial Plating • Polyacrylamide Gel Electrophoresis Molecular Biology • Agarose Gel Electrophoresis • DNA PCR / Sequencing • Customer Service • Inventory Management
Responsible and highly motivated professional with solid and diverse experience as Molecular Biologist with excellence in isolating and purifying DNA, bacterial plating, polyacrylamide gel electrophoresis, agarose gel electrophoresis.
Proficient in cell culture growth, media preparation, visible light and UV spectrophotometers, pipetters, micropipetters, power boxes, centrifuges, autoclaves, and Agarose gel electrophoresis of DNA.